The essay deals with features characteristic for the emigration of Czech expatriates abroad, in several migration waves. It records the role of fellow-countrymen associations, the trans-generational transfer of Czech traditions, and the safeguarding of Czech culture among the members of fellow countrymen associations and their descendants round the world. The authors submit descriptive results from a questionnaire research running in the years 2007 and 2008 (stress was put especially on the forms of trans-generational transfer and the influence of original culture on the respondents´ self-conception). The above research confirmed high influence of original cultural roots on the self-conception of Czech expatriates and their descendants, who took part in the research. Simultaneously, it drew attention to natural assimilation resulting in gradual abandonment of practicing the Czech traditional customs and the elements of Czech folk culture. Along with the 'decline' of daily practicing, the trans-generational transfer in families, substituted by fellow-countrymen associations, gradually loses its influence on handing-over of the traditional components. The fellow-countrymen associations provide 'education' and information for those interested (it is more a form of interest group); on the other hand, the expatriates could find here an alternative place to practice the Czech traditions, as mentioned above. Because ethnic identity is an important part of personal identity of an individual, the clubs - next to the family - are important not only for the entire communities but also for the individuals. Support provided by the Czech government to the clubs is therefore a significant contribution to their work.