The study deals with the structure of the Rosenberg Self-Esteem Scale. In a previous research the several samples of the Czech adolescents were obtained, yielding a similar three-factor structure as the exploratory results. The confirmatory factor analysis was conducted on the joint sample (N = 708, 293 boys and 415 girls) and separately on a high school students group (N = 428, 184 boys and 244 girls) and a university students group as well (N = 280, 109 boys and 171 girls). The analyses corroborated the hypothesis about three-factor structure invariant in both groups. These factors are assertion of the positive statements about self, denial of the negative statements about self and a factor of a social comparison.