The study deals with recent changes in the motivation for pursuing non-formal adult education (NFE) in the Czech Republic and the factors behind this. Although the number of people pursuing NFE has been increasing dynamically in recent years, we do not yet have enough knowledge about the reasons adults pursue NFE and the factors that influence it. In this regard, the study aims to identify (1) the main trends in work/non-work, intrinsic, and extrinsic motivations for NFE and (2) the micro- and meso-social determinants that shape these trends. The study is based on a secondary analysis of data from the Adult Education Survey 2011 (N = 2715) and 2016 (N = 4034). The conclusion shows a significant shift from a non-work-oriented to a work-oriented motivation for participating in NFE and fluctuations between intrinsic to extrinsic motivations. The key factors influencing the 'reorientation' of this motivation among working adults are educational attainment, high occupational status, and to some degree also gender and the size of the company a person works in.