The article describes character of activities and thematic areas of Czech non-profit organizations that focus on promoting gender equality and women's rights in 1990s. Beside the women's organizations the attention is paid to identify the other projects aimed at supporting women or gender equality, but addressed by non-profit organizations whose main object of activity is broader. In addition, the article explores the pro-women oriented non-profit organisations in terms of their memberships, organizational background and geographical extent of their activities. The shifts in topics and activities of Czech women's organizations are identified in process of EU accession and particularly due to change of available resources of funding. This process led to marginalization of 'not mainstream' topics, but in other areas it led to an increase of range of actors addressing projects to promote gender issues (mainly in area of improvement of equal opportunities on the labour market).