This study is concerned with the relationship between musical repertoire and worship services in non-Catholic churches. The repertoire of the Czech Reformation retained many traits from Catholic worship services, such as a large share of Gregorian chant (translated into Czech) and of songs related to late Medieval cantiones. Chant and polyphony were entrusted to the schola (choir), while monophonic songs could be sung by the whole congregation. The main part of the article is devoted to non-Catholic liturgical services – two types of mass (Matura and Summa) and several of the canonical hours (Matins, Prime, Vespers, and Compline) – and to forms of vocal music associated with them. Prescribed liturgy for worship services is compared with the preserved musical repertoire with the goal of understanding better the place of paraliturgical forms (motets and songs) in the framework of vocal music used in worship services.