The article aims to describe the mutual political and diplomatic relations between Czechoslovakia and the State of Israel in 1960–1967. The period of the 1960s in Czechoslovakia was characterized by a partial liberalization and relaxation of the communist regime in various areas such as politics, economy and culture and so on. This process culminated in the well-known Prague Spring of 1967–1968. By using concrete examples, the author explains whether these changes were reflected by Czechoslovak diplomacy in its attitude towards Israel in any way. Special attention is given to Czechoslovak views on Adolf Eichmann’s trial in Jerusalem in 1962, and to the events of the Six Days War in 1967 that led to the official dissolution of Czechoslovak-Israeli diplomatic relations.