The cultural traditions of Gdańsk have been developing since Reformation times. Among the very important features of this city have always been multilingualism, multiculturalism and multiconfessionalism. One of the most important religions in Gdańsk was Protestantism. Since the Reformation times till the end of the Second World War, Protestant traditions influenced the culture of the city and most of all its music life. The development of the music culture in Gdańsk is well-documented in the article. Among the most important sources of Protestant pieces of music from the 16th till 18th century quoted by the author, there are Gdańsk Tablature (Tabulatura Gdańska), Organ tablature from The Cathedral in Gdańsk Oliva (Oliwskiej Tabulaturze Organowej), and Musicalia Gedanenses, which can be found in Staatsbibliothek in Berlin Preußischer Kulturbesitz. recently published by D. Szlagowska, B. Długońska, D. Popinigis, J. Woźniak in Music Collections from Gdańsk (Vol. 1-3, 2007-2011).