One of prerequisites of effective cooperation among the EU Member States in the area of criminal law is not only the theoretical definition, but also the legal regulation of exact procedures, methods and conditions of this cooperation. These intentions resulted in the Act No. 650/2005 Coll. on the execution in the European Union of orders for freezing of property and evidence and on amendment of certain acts („Act“), that represents the transposition of the Council Framework Decision 2003/577/SVV of 22 July 2003 on the execution in the European Union of orders to freeze property and evidence („Framework Decision“) into the national law of the Slovak Republic. This instrument has become the legislative basis and prerequisite of effective cooperation of the individual States in criminal matters with the aim to simplify the process of taking evidence in criminal proceedings. In her article the author refers to the individual conditions and procedures for successful application of this instrument in the practice and in the conclusion evaluated, from her point of view and on the basis of available findings, the usage rate of this instrument in the conditions of the Slovak Republic.