This article deals with a legacy report of six books to the Augustinian Canon monastery in Třeboň which was written down in the years 1460–1468 by Martin of Třeboň, a physician. Two of the manuscripts were identified in the holding of the National Library of the Czech Republic today and moreover, further manuscripts belonging to Martin not mentioned in the legacy were found. They are also held by the National Library of the Czech Republic and by the National Library in Vienna. Another codex belonging to the same owner may be held by the Biblioteca Apostolica Vaticana. The contents of the manuscripts indicate that Martin intended to build up his library as an expert reference library with special regard to medicine and natural sciences but it also contained manuscripts of other branches.