Nitra has a special status in assessment of the liturgical musical codices from the territory of Slovakia dating from the medieval period and early modern period. Only one liturgical manuscript has been preserved in its original place from Nitra’s church institutional funds– the Nitra Codex from the 12th century (ekphonetic symbols, elements of German unlined neume notation). Religious books with notation have not been preserved from the subsequent period (12th to 16th century). All the more surprising, then, is the discovery of 7 antiphonary fragments from the turn of the 16th century with the Estergom notation system in the Nitra Diocesan Archive, which is one of the oldest church archives in the Slovak Republic. On account of the historic status of the Nitra diocese and its church archive, we regard the finding of these fragments as an exceptional discovery in the field of medieval musical research in Slovakia.