Purpose: The aim of the study was to assess the prevalence of human papillomavirus (HPV) DNA in colorectal adenocarcinoma in patients from the Podlasie region undergoing surgery for the tumor. Materials and methods: We examined 40 solid colorectal tumors taken during surgical treatment at the 2nd Department of General and Gastrointestinal Surgery, Medical University of Białystok. HPV was detected by means of polymerase chain reaction (PCR) and in situ hybridization (ISH). The tests were performed on formalin-fixed, paraffin-embedded tumor tissue. Two pairs of primers were used for the detection of HPV DNA by PCR. Pair pU-1M/pU-2R enables detection and identification of high-risk HPV (HPV 16, 18, 31, 33, 35, 52b, 58), while pair pU-31B/pU-2R enables detection and identification of low-risk HPV (HPV 6, 11). The ISH was performed with the use of biotin-labelled dsDNA probes, using Wide Spectrum HPV DNA Probe Cocktail Biotinylated kit, DAKO Cyto-mation. Results: HPV DNA was found in 21 (52.5%) of the examined colorectal tumors. The PCR revealed the presence of viral DNA in 19 (47.5%) tumors. The ISH revealed the presence of HPV DNA in 16 (40%) of the examined tumors. Conclusion: The findings of this study correlate with similar results conducted by other research groups. However, this is the first study of colorectal tumor samples taken from patients of the Podlasie region. Therefore, the association between environmental factors, HPV infection, and tumor stage should also be verified in a larger study population. Further studies confirming the presence of HPV DNA in colorectal tumor tissue in populations from different regions of Poland are needed.