Salivary hexosaminidase B in children with type 1 diabetes

• Authors: Zalewska-Szajda, B , Chojnowska, S , Waszkiewicz, N , Gościk, E , Łebkowska, U , Kępka, A , Bossowski, A , Kuźmiuk, A , Mierzyńska, K , Szajda, SD , Ładny, JR

Abstracts

EN

Introduction: Children type 1 diabetes is accompanied by inflammation and microangiopathy pro-ceeded with increased degradation of tissues. Nacetyl-β-hexosaminidase (HEX) is the most active of exoglycosidases degrading oligo-saccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). Purpose: To evaluate the hexosaminidase B (HEX B) activity in the saliva of children with type 1 diabetes. Materials and methods: The study was performed in 35 children with type 1 diabetes and 20 healthy children. Salivary HEX B activity was determined by the colorimetric, and protein by bicinchoninic acid methods. The HEX B activity concentration was expressed in pKat/mL and specific activity in pKat/µg of protein. Results: A significant increase in the concentration and the specific activity of HEX B in the saliva of children with type 1 diabetes, as compared to healthy children, was found. Conclusion: Children suffering from type 1 diabetes have increased catabolism of salivary gly-coconjugates by HEX B, which potentially may be useful in the diagnosis of type 1 diabetes in children.

Keywords

EN

hexosaminidase isoenzyme B (HEX B); type 1 diabetes; children; saliva

• Publisher: Uniwersytet Medyczny w Białymstoku
• Journal: Progress in Health Sciences
• Year: 2012
• Volume: 2
• Issue: 2
• Pages: null-140

Contributors

author

Zalewska-Szajda, B

author

Chojnowska, S

author

Waszkiewicz, N

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Gościk, E

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Łebkowska, U

author

Kępka, A

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Bossowski, A

author

Kuźmiuk, A

author

Mierzyńska, K

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Szajda, SD

author

Ładny, JR

Identifiers

DOI

143