Zastosowanie elektroforezy do analizy spoiw

• Authors: Burski Zbigniew , Grudzińska Ewa

Title variants

EN

THE USE OF ELECTROPHORESIS IN THE ANALYSIS OF BINDING AGENTS

• Languages of publication: PL

Abstracts

EN

The authors of the present work have carried out studies on the use of disc electrophoresis on polyacrylamid gel to analyze protein binders. Standard electrophoregrams were made of seven binders (hide glue, gelatine, sturgeon glue, egg white, egg yolk, glue from oats and from rye flour). The proteins were then subjected to aging by means of UV radiation, oxygen and temperature. The results obtained have shown the usefulness of the examined method as a way of identifying protein binders. The only identification problem was to differentiate hide glue from gelatine, although this difficulty was due to the fact that an initial protein was collagen from mammals. In the majority of cases, disc electrophoresis on polyacrylamid gel has been found very useful. One of the biggest advantages of that method is the confidence in identification and small quantities of samples necessary to make the analysis (from 20 to 50 mg of protein). It may be believed that this method shall become a compromise between the interests of conservators and of analytical chemists.

Keywords

PL

elektroforeza; analiza spoiw malarskich; analiza elektroforetyczna; spoiwa malarskie

• Publisher: Narodowy Instytut Dziedzictwa
• Journal: Ochrona Zabytków
• Year: 1981
• Issue: 3-4
• Pages: 201-202

Dates

published

1981

Contributors

author

Burski Zbigniew

• dr inż., PP PKZ — Oddział we Wrocławiu

author

Grudzińska Ewa

• mgr inż., Instytut Technologii Przemysłu Chemicznego i Spożywczego Akademii Ekonomicznej we Wrocławiu

References

• J. L e h m a n n , Chemia malarstwa i jego konserwacji, „Biblioteka Muzealnictwa i Ochrony Zabytkow” , seria B, Warszawa 1974.
• J. H o p l i ń s k i , Farby i spoiwa malarskie, „Ossolineum” , 1959.
• Wł. O s t r o w s k i , Elektroforeza w badaniach chemicznych i klinicznych, PWN, Warszawa 1970.
• R. С hu, Identification o f Commercially Used Fish Found in the Pacific by Disk Electrophoresis, ,,J. Ass. Offic. Agr. Chem.” , 51, 1968, s. 743;
• Y. B. L e e D.A. R i c a n d s r u d , E.C. H a g b e r g , E. I. Br i s k e y , Quantitative Determination o f Soybean Protein in Fresh and Cooked Meat-Soy Blends, ,,J. of Food Sei.”, 40,1975,s. 380;
• Y. B. Lee, D. A. R i с a n d sr u d, E. C. Ha gb e r g , R. H. F o r s y t h e , Detection o f Various Nonmeat Extenders in Meat Produets, ,,J. of Food Sei.”, 41, 1976, s. 589;
• E. S p e l l , Die Elektrophoretische Unterscheidung Verschiedener Fleischarten, „Die Fleischwirtschaft”, 54, 1974, s. 533;
• E. Spe l l , Nachweis von Milcheiweiss und Sojaeiweiss in Fleischerzeugnissen mit H ilf der Vertikalen Flochdisk-elektrophorese, „Die Fleischwirtschaft”, 52, 1972, s. 1451.
• W. S. H i l l, R. J. L e a r s o n , J. P. L a n e, Identification o f Fish Species by Agar Gel Electrophoresis, „J. Ass. Offic. Agr. Chem.”, 49, 1966, s. 1245.
• R. R. T h o m p s o n , Species Identification by Starch Gel Zone Electrophoresis o f Protein Extracts. /. Fish, „J. Ass. Offic. Agr. Chem.”,
• J. K о h n, A New Supporting Medium for Zone Electrophoresis, „Biochem. J.” , 65, 1957, s. 9.