Evaluation of neutrophil phagocytic, complement functions, and cytokines expression among diabetic patients in Abuja, Nigeria

• Authors: Musa Muhammad Babandina , Abdullahi Suleiman Mainasara , Mustapha Bakare , Anthony Uchenna Emeribe , Halima Ali Shuwa , Shamsuddeen Haruna , Aminu Said Muhammad , Idris Nasir Abdullahi
• Languages of publication: EN

Abstracts

EN

Introduction. Inflammatory response in Diabetes Mellitus (DM) begins with chronic sub-clinical inflammations as a result of insulin resistance and activation of both innate and adaptive immune system as the disease progresses to complicated diabetes. Hence, the present study investigated the neutrophil phagocytic, complement function (CH50), and some cytokine profiles among diabetic and non-diabetic patients attending the National Hospital in Abuja, Nigeria. Aim. To evaluate the neutrophil phagocytic, complement function (CH50), and some cytokine profiles among post-operative septic diabetic and post-operative septic non-diabetic patients at the National Hospital in Abuja, Nigeria. Material and methods. Subjects were recruited by convenient sampling technique through interviewer-administered questionnaires. Subsequently, blood samples were collected. Fasting blood sugar (FBS) (mmol/L) was determined using glucose oxidase method. Neutrophil function test (Fmol/phag) was assayed using nitroblue tetrazolium reduction test (NBT). Hemolytic complement function (CH 50) test was conducted using serum harvested from sheep sensitized with human group (ORh D +ve) red blood cells. While serum Interleukin-4, -6, -10 and TNF- α were determined using Enzyme Linked Immunosorbent Assay (ELISA). Results. Mean ± Standard deviation (SD) of FBS concentration of 10.5 ± 1.3 (mmol/|L) among diabetic and 4.7 ± 0.9 (mmol/L) among non-diabetics was recorded. There is a decrease in neutrophil phagocytic function with a mean ± SD of 5.4 ± 2.1 (Fmol/ phag) in diabetics compared to 9.2 ± 2.1 (Fmol/phag) in non-diabetics. Similarly, complement (CH 50) function and C-reactive protein were significantly lower in diabetics when compared to non-diabetics (p<0.001). There was a significant difference in IL-6 concentration between diabetics and non-diabetics groups, but no significant difference was observed in TNF-α, IL-4 and IL-10 concentrations between study groups (p>0.05). TNF-α and IL-6 was significantly higher in diabetics with cardiovascular disorders compared to non-diabetics subjects with cardiovascular disorders (p<0.001). Conclusion. Findings from this study revealed the association of complement, neutrophil phagocytic function, CRP and IL-6 among septic diabetic patients,. In addition TNF-α and IL-6 expression was higher in DM patients with cardiovascular disorders.

Keywords

EN

diabetes mellitus; effector molecules; pro-inflammatory markers

• Publisher: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
• Journal: European Journal of Clinical and Experimental Medicine
• Year: 2019
• Issue: 3
• Pages: 229-235

Contributors

author

Musa Muhammad Babandina

• Department of Medical Laboratory Services, University of Abuja Teaching Hospital Gwagwalada, FCT Abuja, Nigeria

author

Abdullahi Suleiman Mainasara

• Department of Chemical Pathology, School of Medical Laboratory Sciences, Usmanu Danfodiyo University Sokoto, Sokoto, Nigeria

author

Mustapha Bakare

• Department of Medical Laboratory Services, University of Abuja Teaching Hospital Gwagwalada, FCT Abuja, Nigeria

author

Anthony Uchenna Emeribe

• Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria

author

Halima Ali Shuwa

• Department of Community Health, Federal University Dutse, Jigawa, Nigeria

author

Shamsuddeen Haruna

• Department of Medical Laboratory Science, Ahmadu Bello University, Zaria, Nigeria

author

Aminu Said Muhammad

• Department of Medical Laboratory Science, Ahmadu Bello University, Zaria, Nigeria

author

Idris Nasir Abdullahi

• Department of Medical Laboratory Science, Ahmadu Bello University, Zaria, Nigeria

Identifiers

DOI

10.15584/ejcem.2019.3.5