Reverse phase high performance liquid chromatographic method development based on ultravioletvisible detector for the analysis of 1-hydroxypyrene (PAH biomarker) in human urine

• Authors: Atif Kamal , Mohammad Gulfraz , Mohammad A. Anwar , Riffat N. Malik

Abstracts

EN

Objectives 1-hydroxypyrene is an important biomarker of exposure to polycyclic aromatic hydrocarbons (PAHs), which appears in the urine of exposed human subjects. In developing countries, where advanced instruments are not available, the importance of this biomarker demands convenient and sensitive methods for determination purposes. This study aimed at developing a methodology to quantify 1-hydroxypyrene (a biomarker of PAHs exposure) based on the UV-visible detector in the reverse phase high pressure liquid chromatography (HPLC). Material and Methods A 20 μl injection of sample was used for manual injection into the HPLC Shimadzu, equipped with the SPD-20 A UV-visible detector, the LC-20AT pump and the DGU-20A5 degasser. The C-18 column was used for the purpose of the analysis. Results The method showed a good linearity (the range: R² = 0.979–0.989), and high detectability up to the nmol level. The average retention was 6.37, with the accuracy of 2%, and the percentage of recovery remained 108%. The overall performance of this method was comparable (in terms of detection sensitivity) and relatively better than previously reported studies using the HPLC system equipped with the UV-detector. Conclusions This method is suitable and reliable for the detection/quantification of the 1-OHP in human urine samples, using the UV-detector, however, it is less sensitive as compared to the results of a florescence detector.

Keywords

EN

1-OHP; 1-hydroxypyrene; high pressure liquid chromatography; Pakistan; biomarkers of exposure; PAHs

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2015
• Volume: 28
• Issue: 2
• Pages: 399-403

Dates

published

2015

Contributors

author

Atif Kamal

• Quaid-i-Azam University, Islamabad, Pakistan (Faculty of Biological Sciences, Department of Environmental Sciences, Environmental Biology and Ecotoxicology Laboratory)

author

Mohammad Gulfraz

• Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan (Institute of Biochemistry)

author

Mohammad A. Anwar

• Pir Mehr Ali Shah, Arid Agriculture University, Rawalpindi, Pakistan (Institute of Biochemistry)

author

Riffat N. Malik

• Quaid-i-Azam University, Islamabad, Pakistan (Faculty of Biological Sciences, Department of Environmental Sciences, Environmental Biology and Ecotoxicology Laboratory)

Identifiers

DOI

10.13075/ijomeh.1896.00325

Biblioteka Nauki

2177414