Sperm quality and DNA integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons

• Authors: Hueiwang Anna Jeng , Chih-Hong Pan , Mu-Rong Chao , Chien-Chih Chiu , Guodong Zhou , Chon-Kit Chou , Wen-Yi Lin

Abstracts

EN

Objectives The objective of this study was to assess sperm quality and deoxyribonucleic acid (DNA) integrity of coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs) as compared to control subjects. Material and methods The coke oven workers (N = 52) and administrative staff (N = 35) of a steel plant served as the exposed and control groups, respectively. Exposure to PAHs was assessed by measuring 1-hydroxypyren. Analysis of sperm quality (concentration, motility, vitality, and morphology) was performed simultaneously with sperm DNA integrity analysis, including DNA fragmentation, denaturation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo). A questionnaire was conducted to collect demographic and potential confounding data. Results The coke oven workers had lower percentages of sperm motility, vitality and normal morphology than the control group, but the difference was not significant. For DNA integrity, the coke oven workers had significantly higher concentrations of bulky DNA adducts and 8-oxo-dGuo than the control subjects (p = 0.009 and p = 0.048, respectively). However, DNA fragmentation percentages did not significantly increase as compared to those in the subjects from the control group (p = 0.232). There was no correlation between sperm quality parameters and DNA integrity indicators. Conclusions Occupational exposure of the coke oven workers to PAHs was associated with decreased sperm DNA integrity. Int J Occup Med Environ Health 2016;29(6):915–926

Keywords

EN

DNA damage; semen quality; sperm; DNA integrity; DNA fragmentation; bulky DNA adducts

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2016
• Volume: 29
• Issue: 6
• Pages: 915-926

Dates

published

2016

Contributors

author

Hueiwang Anna Jeng

• Old Dominion University, Norfolk, USA (College of Health Sciences, School of Community and Environmental Health)

author

Chih-Hong Pan

• Council of Labor Affairs, Executive Yuan, Taipei, Taiwan (Institute of Occupational Safety and Health)

author

Mu-Rong Chao

• Chung Shan Medical University, Taichung, Taiwan (Department of Occupational Safety and Health)

author

Chien-Chih Chiu

• Kaohsiung Medical University, Kaohsiung, Taiwan (College of Health Sciences, Department of Medical Laboratory Science and Biotechnology)

author

Guodong Zhou

• Texas A&M Health Science Center, Houston, USA (Institute of Biosciences and Technology, Center for Cancer and Stem Cell Biology)

author

Chon-Kit Chou

• Kaohsiung Medical University, Kaohsiung, Taiwan (College of Health Sciences, Department of Medical Laboratory Science and Biotechnology)

author

Wen-Yi Lin

• Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan (Department of Occupational Medicine and Internal Medicine)

Identifiers

DOI

10.13075/ijomeh.1896.00598

Biblioteka Nauki

2168377