Pathogenic and phylogenetic features of 2 multiresistant Pseudomonas aeruginosa strains originated from remediated sites

• Authors: Edit Kaszab , Sándor Szoboszlay , Gyula Dura , Júlia Radó , Balázs Kovács , Balázs Kriszt

Abstracts

EN

Objectives To evaluate the possible occupational hazard of environmental strains of opportunistic Pseudomonas aeruginosa on hydrocarbon contaminated sites during remediation, 2 multidrugresistant isolates originating from environmental (soil and groundwater) samples were examined. Material and Methods Antibiotic resistance profiles of the examined 2 strains were determined by Etest® against 20 different agents. Virulence investigations included the hemolytic activity test, the detection of virulence-related gene sequences such as exoA, exoU, exoS, exoY, exoT and the determination of intraperitoneal LD 50 (the lethal dose, 50%) values in a mouse model. The hydrocarbon-degrading ability was evaluated in a gravimetric experiment, in vitro. The phylogenetic relationship of the isolates was investigated with a multilocus sequence typing scheme. Results Multidrug resistant environmental strains of P. aeruginosa are strongly related to isolates that have proven effects on the infection of patients who suffer from cystic fibrosis, have a notable hemolytic activity, carry important virulence markers (exoS or exoU, respectively) and retain their hydrocarbon degradation ability (87.4% and 62.8% hydrocarbon degradation rate, respectively). Conclusions Pseudomonas aeruginosa presumably raise considerable concerns for human health in the environment, already well known among nosocomial isolates, and the application of environmental strains of this species for environmental purposes is questionable.

Keywords

EN

Pseudomonas aeruginosa; pathogenicity; MLST; antibiotic resistance; hydrocarbon degradation; remediation

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2015
• Volume: 29
• Issue: 3
• Pages: 503-516

Dates

published

2016

Contributors

author

Edit Kaszab

• Szent István University, Gödöllő, Hungary (Department of Environmental Safety and Ecotoxicology)

author

Sándor Szoboszlay

• Szent István University, Gödöllő, Hungary (Department of Environmental Safety and Ecotoxicology)

author

Gyula Dura

• National Institute of Environmental Health, Budapest, Hungary

author

Júlia Radó

• Szent István University, Gödöllő, Hungary (Department of Environmental Safety and Ecotoxicology)

author

Balázs Kovács

• Szent István University, Gödöllő, Hungary (Department of Aquaculture)

author

Balázs Kriszt

• Szent István University, Gödöllő, Hungary (Department of Environmental Safety and Ecotoxicology)

Identifiers

DOI

10.13075/ijomeh.1896.00679

Biblioteka Nauki

2177448