Effects of benzo(a)pyrene exposure on the ATPase activity and calcium concentration in the hippocampus of neonatal rats

• Authors: Kai Yang , Chengzhi Chen , Shuqun Cheng , Xianqing Cao , Baijie Tu

Abstracts

EN

Objectives To investigate whether postnatal benzo(a)pyrene (B(a)P) exposure caused the impairments on the process of neurodevelopment and the alteration in the calcium medium in the neonatal rats. Material and Methods Eighty neonatal Sprague Dawley (SD) rats were randomly divided into 5 groups (untreated control group, vehicle group, 0.02 mg/kg, 0.2 mg/kg and 2 mg/kg B(a)P-exposed group). Rats were treated with B(a)P by the intragastric administration from postnatal day (PND) 4 to 25. Morris water maze (MWM) was employed to observe the spatial memory of rats. The activity of calcium adenosine triphosphatase (Ca²⁺-ATPase), sodium-potassium adenosine triphosphatase (Na⁺-K⁺-ATPase) and calcium-magnesium adenosine triphosphatase (Ca²⁺-Mg²⁺-ATPase) in the hippocampus were detected by commercial kits. Fura-2 pentakis(acetoxymethyl) (Fura-2/AM) probe and reactive oxygen species (ROS) reagent kit were used for measuring the concentration of Ca²⁺ and ROS in the hippocampus synapse, respectively. Results Rats exposed to B(a)P resulted in the deficits in the spatial memory manifested by the increased escape latency and decreased number of crossing platform and time spent in target quadrant in comparison with the control groups. Benzo(a)pyrene exposure caused the significant decrease in the ATPase activity in the hippocampus and caused Ca²⁺ overload in the synaptic, besides, the ROS concentration increased significantly which may further induce neurobehavioral impairment of the neonatal rats. Conclusions Our findings suggest that postnatal B(a)P exposure may cause the neurobehavioral impairments in the neonatal rats, which were mediated by the decreased ATPase activity and elevated Ca²⁺ concentration. Int J Occup Med Environ Health 2017;30(2):203–211

Keywords

EN

hippocampus; neurodevelopment; ATPase activity; Ca2+ concentration; neonatal rats; Benzo(a)pyrene

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2017
• Volume: 30
• Issue: 2
• Pages: 203-211

Dates

published

2017

Contributors

author

Kai Yang

• Chongqing Medical University, Chongqing, China (Innovation Center for Social Risk Governance in Health, Research Center for Medicine and Social Development, School of Public Health and Management, Department of Occupational and Environmental Health)

author

Chengzhi Chen

• Chongqing Medical University, Chongqing, China (Innovation Center for Social Risk Governance in Health, Research Center for Medicine and Social Development, School of Public Health and Management, Department of Occupational and Environmental Health)

author

Shuqun Cheng

• Chongqing Medical University, Chongqing, China (Innovation Center for Social Risk Governance in Health, Research Center for Medicine and Social Development, School of Public Health and Management, Department of Occupational and Environmental Health)

author

Xianqing Cao

• Chongqing Medical University, Chongqing, China (Center of Experimental Teaching for Public Health, Experimental Teaching and Management Center)

author

Baijie Tu

• Chongqing Medical University, Chongqing, China (Innovation Center for Social Risk Governance in Health, Research Center for Medicine and Social Development, School of Public Health and Management, Department of Occupational and Environmental Health)

Identifiers

DOI

10.13075/ijomeh.1896.00744

Biblioteka Nauki

2161850