Albumin adducts and urinary metabolites resulting from occupational exposure to 1,5-naphthalene diisocyanate

• Authors: Ovnair Sepai , Gabriele Sabbioni

Abstracts

EN

Objectives 1,5-Naphthalene diisocyanate (NDI) is used in the plastic industry as a curing agent. 1,5-Naphthalene diisocyanate is classified as a sensitizing agent. The objective of this study has been to develop biomonitoring methods for the evaluation of exposure to NDI. Material and Methods We obtained blood and urine samples from a group of 20 male workers exposed to NDI. The workers answered a questionnaire about their exposure history, job description, the number of years with the company and the time spent working with NDI over the 10 days of the study. Total plasma, albumin, and urine were analyzed for the presence of 1,5-naphthalenediamine (NDA) after acid hydrolysis using gas chromatography-mass spectrometry (GC-MS). Results 1,5-Naphthalenediamine was found in about 60% of the samples obtained from the workers. 1,5-Naphthalenediamine was obtained after acid hydrolysis of plasma, albumin, and urine at levels up to 1.5 pmol NDA/mg of plasma proteins, 1.15 pmol NDA/mg of albumin, and 55.3 pmol NDA/ml of urine, respectively. Conclusions 1,5-Naphthalenediamine found in urine correlates best with the plasma levels (r = 0.91, p < 0.01). The albumin-adduct levels did not correlate with the NDI-specific immunoglobulin E (IgE) or total IgE present in the workers. The adduct and metabolite levels correlate with the air levels of NDI. Int J Occup Med Environ Health 2017;30(4):579–591

Keywords

EN

diisocyanates; biomonitoring; urinary metabolites; albumin adducts; arylamine; occupational hygiene

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2017
• Volume: 30
• Issue: 4
• Pages: 579-591

Dates

published

2017

Contributors

author

Ovnair Sepai

• University of Würzburg, Würzburg, Germany (Department of Toxicology)

author

Gabriele Sabbioni

• University of Würzburg, Würzburg, Germany (Department of Toxicology)
• Institute of Environmental and Occupational Toxicology, Airolo, Switzerland
• University of Munich, Munich, Germany (Walther-Straub-Institute of Pharmacology and Toxicology)

Identifiers

DOI

10.13075/ijomeh.1896.00863

Biblioteka Nauki

2161899