Evaluation of circulating microparticles in healthy medical workers occupationally exposed to ionizing radiation: A preliminary study

• Authors: Ghassan Al-Massarani , Fadi Najjar , Abdulmunim Aljapawe , Adnan Ikhtiar

Abstracts

EN

Objectives Ionizing radiation was known to cause disruption of cytoskeleton. However, the disorganization of the cytoskeleton leads to form microparticles (MP) that carry membrane and cytoplasmic constituents from their parent cells they are released from. Therefore, authors investigated the effect of the occupational exposure to low doses of ionizing radiation on MP levels. Material and Methods The current study was conducted on 38 healthy medical workers occupationally exposed to low doses of ionizing radiation and 29 controls matched by gender, age, and smoking habits. The MP levels measured by flow cytometry were classified as positive or negative phosphatidylserine (PS⁺ or PS⁻), and phenotyped according to their cellular origin. Results Total MP (PS–/PS+) levels, regardless of phenotype, were significantly higher in workers occupationally exposed to ionizing radiation than in healthy individuals (p = 0.0004). Negative phosphatidylserine microparticles were predominant in medical exposed workers and, to a lesser extent, in controls (68% and 57%, respectively). With regard to phenotypic characterization of cellular origin, MP derived from platelets (CD41a+), endothelial (CD146+), leucocytes (CD45+) and erythrocytes (CD235a+) MP were significantly enhanced in exposed workers compared with controls (p < 0.0001). However, no significant difference was found in the proportion of the other blood elements in the peripheral circulation between the 2 groups. Serum levels of C-reactive protein were normal for all individuals. In addition, no association was observed between MP levels and the studied confounding factors. Conclusions The results suggest that elevated circulating MP levels represent an indicator of cellular damage caused by medical exposure to low doses of ionizing radiation. By consequence, the quantification of MP seems to be a useful biomarker for assessing the negative effects of occupational exposure to ionizing radiation. Int J Occup Med Environ Health 2018;31(6):783–793

Keywords

EN

biomarkers; flow cytometry; occupational exposure; microparticles; ionizing radiation; cellular damage

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2018
• Volume: 31
• Issue: 6
• Pages: 783-793

Dates

published

2018

Contributors

author

Ghassan Al-Massarani

• Atomic Energy Commission of Syria (AECS), Damascus, Syria (Biomarkers Laboratory, Department of Radiation Medicine)

author

Fadi Najjar

• Atomic Energy Commission of Syria (AECS), Damascus, Syria (Biomarkers Laboratory, Department of Radiation Medicine)

author

Abdulmunim Aljapawe

• Atomic Energy Commission of Syria (AECS), Damascus, Syria (Radiobiology Laboratory, Department of Molecular Biology and Biotechnology)

author

Adnan Ikhtiar

• Atomic Energy Commission of Syria (AECS), Damascus, Syria (Radiobiology Laboratory, Department of Molecular Biology and Biotechnology)

Identifiers

DOI

10.13075/ijomeh.1896.01106

Biblioteka Nauki

2162036