Metabolism and in vitro assessment of the mutagenic activity of urinary extracts from rats after inhalation exposure to 1-methylnaphthalene

• Authors: Radosław Świercz , Maciej Stępnik , Jolanta Gromadzińska , Katarzyna Domeradzka-Gajda , Joanna Roszak , Wojciech Wąsowicz

Abstracts

EN

Objectives 1-Methylnaphthalene (1-MN) is composed of 2 benzene rings and belongs to polycyclic aromatic hydrocarbons. The metabolism of 1-MN in laboratory animals and bacteria leads to the formation of 1-naphthoic acid (1-NA). Material and Methods In this study the distribution of 1-NA in lung, liver, spleen, kidney and urinary excretion of 1-NA in rats after single and repeated inhalation exposure to 1-MN vapors were investigated. The activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and cytochrome were measured of the rats. Genotoxic effects were evaluated with the in vitro micronucleus test on V79 hamster fibroblasts. Results The concentrations of 1-NA in the tissues of rats after single and repeated exposure to 1-MN were dependent on the exposure dose. High levels of 1-NA were found in kidneys of animals after the single and repeated exposure to 1-MN. With an increase of 1-MN dose, an increase in the activity of cytochrome P450 (CYP1A1 and CYP1A2) was observed in the liver of rats. Compared to control animals, significantly higher ALT activity was noted in serum of rats exposed to 1-MN. The micronuclei frequency in V79 cells exposed to 1-MN (in the range of analyzable concentrations; i.e., 5–25 μg/ml) did not differ significantly from the vehicle control, whereas urine extracts from rats exposed to 1-MN induced a significant increase in the frequency of micronuclei compared to urine extracts from the group of control animals. Conclusions Metabolism of 1-MN in rats after the inhalation exposure leading to 1-NA was mainly observed during the first day after the end of exposure. It is likely that 1-MN metabolites present in rat urine can induce the increased micronuclei frequency as was shown in V79 cells.

Keywords

EN

rats; inhalation; micronucleus test; toxicokinetics; 1-methylonaphthalene; 1-naphthoic acid

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2022
• Volume: 35
• Issue: 6
• Pages: 731-746

Dates

published

2022

Contributors

author

Radosław Świercz

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)

author

Maciej Stępnik

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)
• QSAR Lab, Ltd, Gdańsk, Poland

author

Jolanta Gromadzińska

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Biological and Environmental Monitoring)

author

Katarzyna Domeradzka-Gajda

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)

author

Joanna Roszak

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Translational Research)

author

Wojciech Wąsowicz

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Biological and Environmental Monitoring)

Identifiers

DOI

10.13075/ijomeh.1896.01955

Biblioteka Nauki

2152982