The use of LA-ICP-MS as an auxiliary tool to assess the pulmonary toxicity of molybdenum(IV) sulfide (MoS₂) nano- and microparticles

• Authors: Renata Kuraś , Maciej Stępnik , Katarzyna Domeradzka-Gajda , Beata Janasik

Abstracts

EN

Objectives Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has considerable applicative potential for both qualitative and quantitative analyses of elemental spatial distribution and concentration. It provides high resolutions at pg-level detection limits. These qualities make it very useful for analyzing biological samples. The present study responds to the growing demand for adequate analytical methods which would allow to assess the distribution of nanostructured molybdenum(IV) disulfide (MoS₂) in organs. It was also motivated by an apparent lack of literature on the biological effects of MoS₂ in living organisms. The study was aimed at using LA-ICP-MS for comparing micro- and nanosized MoS₂ ditribution in selected rat tissue samples (lung, liver, brain and spleen tissues) after the intratracheal instillation (7 administrations) of MoS₂ nano- and microparticles vs. controls. Material and Methods The experimental study, approved by the Ethics Committee for Animal Experiments was performed using albino Wistar rats. This was performed at 2-week intervals at a dose of 5 mg/kg b.w., followed by an analysis after 90 days of exposure. The MoS₂ levels in control tissues were determined with the laser ablation system at optimized operating conditions. The parameter optimization process for the LA system was conducted using The National Institute of Standards and Technology (NIST) glass standard reference materials. Results Instrument parameters were optimized. The study found that molybdenum (Mo) levels in the lungs of microparticle-exposed rats were higher compared to nanoparticle-exposed rats. The opposite results were found for liver and spleen tissues. Brain Mo concentrations were below the detection limit. Conclusions The LA-ICP-MS technique may be used as an important tool for visualizing the distribution of Mo on the surface of soft samples through quantitative and qualitative elemental mapping.

Keywords

EN

microparticles; LA-ICP-MS; molybdenum(IV) disulfide; bioimaging; rat tissues; nanoparticles

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2023
• Volume: 37
• Issue: 1
• Pages: 18-33

Dates

published

2024

Contributors

author

Renata Kuraś

• Nofer Institute of Occupational Medicine, Łódź, Poland (Central Laboratory)

author

Maciej Stępnik

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Toxicology and Carcinogenesis)
• QSAR LAB Ltd., Gdańsk, Poland

author

Katarzyna Domeradzka-Gajda

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Toxicology and Carcinogenesis)

author

Beata Janasik

• Nofer Institute of Occupational Medicine, Łódź, Poland (Department of Chemical Safety)

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Identifiers

DOI

10.13075/ijomeh.1896.02305

Biblioteka Nauki

28761978