Effects of hypericin-mediated photodynamic therapy on GM-CSF, MIF, VCAM-1 and ICAM-1 secretion in colorectal cancer cells in vitro

• Authors: Anna Międzybrodzka , Aleksandra Kawczyk-Krupka , David Aebisher , Grzegorz Cieślar , Zenon Paweł Czuba

Abstracts

EN

Introduction and aim. Photodynamic therapy with hypericin (HYP-PDT) is gaining importance as a potential treatment method for malignant neoplasms. The following study investigated whether HYP-PDT influences the secretion of certain factors of colon cancer cells in vitro. Material and methods. Two colon cancer cell lines were used in this experiment: SW480 and SW620. The cells were properly prepared and then treated with photodynamic therapy with hypericin at concentrations of 0.25 µM and 0.5 µM and irradiation at the doses of 1 J/cm2 , 5 J/cm2 and 10 J/cm2 . After using HYP-PDT, changes in the concentrations of four factors: GM-CSF, MIF, VCAM-1 and ICAM-1 were analyzed in the test tubes. Results. In the case of SW480 cells: a notable decrease in GM-CSF, MIF, VCAM-1 and ICAM-1 secretion was noted after HYP-PDT. In the case of SW620 cells, after HYP-PDT: no effect on GM-CSF secretion was noted; it inhibited the secretion of VCAM-1 and MIF and increased the secretion of ICAM-1. Conclusion. Photodynamic therapy with hypericin shows immunomodulatory potential in an in vitro cell line experiments. This may indicate its possible ability to modify the course of malignant tumors and therefore requires further research.

Keywords

EN

colorectal cancer; hypericin; photodynamic therapy

• Publisher: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
• Journal: European Journal of Clinical and Experimental Medicine
• Year: 2023
• Issue: 2
• Pages: 202-216

Dates

published

2023

Contributors

author

Anna Międzybrodzka

• Department of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

Aleksandra Kawczyk-Krupka

• Department of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

David Aebisher

• Department of Photomedicine and Physical chemistry, Faculty of Medicine, University of Rzeszow, Rzeszow, Poland

author

Grzegorz Cieślar

• Department of Internal Medicine, Angiology and Physical Medicine, Center for Laser Diagnostics and Therapy, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

author

Zenon Paweł Czuba

• Department of Microbiology and Immunology, School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Zabrze, Poland

Identifiers

DOI

10.15584/ejcem.2023.2.2

Biblioteka Nauki

25102366