PL EN


2015 | 70 | 1 |
Article title

Fluorescencja chlorofilu w odpowiedzi pszenicy na egzogenną aplikację regulatorów wzrostu w stresie suszy

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EN
Abstracts
EN
Drought stress negatively affects plant photosynthesis and disturbs the electron transport activity. Evaluation of the chlorophyll fluorescence parameters might reflect influence of the environmental stress on plants and can be applied as an indicator of the primary photochemistry of photosynthesis. In current study the effect of foliar application of benzylaminopurine (BAP, a synthetic cytokinin) and abscisic acid (ABA) on chlorophyll fluorescence parameters of relatively drought tolerant (Pishtaz) and susceptible (Karaj3) bread wheat genotypes under well watered and terminal water deficit condition have been evaluated. Terminal drought was induced by withholding water at anthesis stage (Zadoks scale 65). Results showed that coefficient of non-photochemical quenching of variable fluorescence (qN), quantum yield of PS II photochemistry (ΦPSII) and photochemical quenching (qP) were affected by hormone spray treatments. So that evaluation of parameters at 7 day after foliar treatments revealed that ABA significantly increased electron transport rate (ETR) and qN while considerably decreased ΦPSII, gs and maximum quantum yield of photosystem II (Fv/Fm). However exogenous application of cytokinin could increase gs, Fv/Fm and ΦPSII and the highest value of these parameters was recorded in cytokinin treated plants of Pishtaze cv. under well watered condition. Nevertheless, evaluation of the parameters in different periods after spraying showed that with approaching the maturity stage some traits like as gs, Fv/Fm and ETR significantly decreased in both genotypes. Evaluation of gs and Chlorophyll fluorescence parameters of genotypes between different irrigation levels showed that although cv. Pishtaz showed higher performance of PSII under well watered condition, it failed to maintain its superiority under stress condition. This finding suggests that some more responsive parameter like gs, Fv/Fm and ΦPSII can be considered as reliable indicator for understanding the biochemical and physiological effects of exogenous application of phytohormones under terminal drought stress.
PL
Stres suszy wpływa negatywnie na fotosyntezę roślin oraz zakłóca transport elektronów. Ocena parametrów fluorescencji chlorofilu może odzwierciedlać wpływ stresu środowiskowego na rośliny i może być stosowana jako wskaźnik pierwotnych reakcji fotochemicznych fotosyntezy. W prezentowanych badaniach oceniano wpływ dolistnego stosowania benzyloaminopuryny (BAP, syntetyczna cytokinina) i kwasu abscysynowego (ABA) na parametry fluorescencji chlorofilu w stosunkowo odpornych na suszę (Pishtaz) i podatnych (Karaj3) genotypach pszenicy w warunkach dobrego nawodnienia i skrajnego deficytu wody. Krańcową suszę wywoływano w fazie kwitnienia (65 w skali Zadoks). Wykazano, że współczynnik niefotochemicznego wygaszania fluorescencji (qN), wydajność kwantowa reakcji fotochemicznych PS II (ΦPSII) i fotochemiczne wygaszanie (qP) ulegały zmianom po opryskiwaniu liści hormonami. Ocena parametrów po 7 dniach od dolistnego podania hormonu wykazała, że ABA znacząco zwiększa sprawność transportu elektronów (ETR) i qN, znacznie zmniejsza ΦPSII, gs i maksymalną wydajność kwantową fotosystemu II (Fv/Fm). Jednak egzogenne zastosowanie cytokininy może zwiększyć gs, Fv/Fm i ΦPSII, a najwyższą wartość tych parametrów odnotowano w traktowanych cytokininą roślinach odmiany Pishtaze w warunkach dobrego nawodnienia. Ocena parametrów w różnych okresach po oprysku wykazała, że w bardziej dojrzałych roślinach wskaźniki takie jak gs, Fv/Fm i ETR znacznie zmniejszyły się w obu genotypach. Ocena gs i parametrów fluorescencji chlorofilu genotypów w warunkach różnych poziomów nawodnienia wykazała, że chociaż genotypy Pishtaz wykazały wyższą wydajność PSII w stanie dobrego nawodnienia, nie udało się utrzymać przewagi w warunkach stresu. Odkrycie to sugeruje, że niektóre bardziej czułe parametry takie jak gs, Fv/Fm i ΦPSII mogą być wiarygodnym wskaźnikiem dla zrozumienia biochemicznych i fizjologicznych efektów egzogennego stosowania fitohormonów w warunkach krańcowego stresu suszy.
Year
Volume
70
Issue
1
Physical description
Dates
published
2015
online
2015-09-07
Contributors
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.ojs-doi-10_17951_c_2015_70_1_13
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