Assessment of 8-oxo-7,8-dihydro-2′-deoxyguanosine as a marker of oxidative DNA damage in gasoline filling station attendants

• Authors: Ravichandran Beerappa , Dhananjayan Venugopal , Somnath Sen , Mala Ambikapathy , Rajmohan H. Rao

Abstracts

EN

Objectives: The urinary excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) was used as a biomarker of oxidative DNA damage. The urinary 8-oxodG levels in petrol filling station attendants (exposed) at various petrol bunks were estimated as well as in the unexposed (cashier) population. Materials and Methods: A total of 100 workers (79 petrol fillers and 21 cashiers) aged from 20 to 41 years participated in the study. An informed consent was taken from each participant. Information on personal habits and health was obtained through a questionnaire. After shifts, urine samples were collected analyzed for 8-oxodG using enzyme-linked immunosorbent assay (ELISA). Results: Fifty-three percent of workers were in the 21-30 years age group. The maximum level of 8-oxodG was observed in the age group ≥ 41 years and the minimum in the age group of 31-40 years. The maximum level of 8-oxodG was observed among those workers who had ≥ 21 years of experience. The concentrations of 8-oxodG were significantly higher in petrol fillers than those in cashiers (p < 0.05). Conclusions: Despite the conflicting results obtained in our study it was shown that 8-oxodG is related to chemical exposure. Further research is needed embracing a bigger number of participants to highlight the correlations between the exposure and the effects.

Keywords

EN

DNA damage; ELISA; petrol filling attendants; urinary 8-oxo-7; 8-dihydro-2’-deoxyguanosine

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2013
• Volume: 26
• Issue: 5
• Pages: 780-789

Dates

published

2013

Contributors

author

Ravichandran Beerappa

• Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India

author

Dhananjayan Venugopal

• Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India

author

Somnath Sen

• Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India

author

Mala Ambikapathy

• Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India

author

Rajmohan H. Rao

• Regional Occupational Health Center (S), Industrial Hygiene & Toxicology Division, Bangalore, India

Identifiers

DOI

10.2478/s13382-013-0149-0

Biblioteka Nauki

2179055