Investigating the anti-cancer properties of Trametes gibbosa extract on human colon cancer cells

• Authors: Barbara Siewert

Title variants

PL

Badanie antykancerogennych właściwości ekstraktu z wrośniaka garbatego (Trametes gibbosa)

Abstracts

EN

Trametes gibbosa, also referred to as lumpy bracket, is a fungus belonging to the Polyporaceae with natural bioactive compounds that have aroused great interest for their potential benefits in human health. This pilot study aimed to investigate the anti-cancer effect of Trametes gibbosa extract against the HCT-116 cell line (human colorectal cancer cells). The extract was obtained by macerating dry biomass with 3 solvents: acetone, isopropyl alcohol and DCM, isolating established anticancer agents –polysaccharide complexes (PSK and PSP) from the extract, and then being dissolved. Cellular toxicity was assessed by trypan blue staining, determining the effect of 1- and 24-hour continuous exposure to the extract of varying concentrations: 100 µl/mL, 200 µl/mL, 500 µl/mL, 1000 µl/ mL. Results have demonstrated a cytotoxic effect in time- and dose-dependent manners. The proposed cellular mechanisms behind the results are most likely apoptosis followed by secondary necrotic cell death, as the literature suggests, although that remains unclear and requires further experiments. Within the course of consecutive studies, it can be established whether the extract or the compounds contained may hold promise for clinical use, e.g., for adjuvant therapy.

Keywords

EN

Trametes gibbosa; colon cancer; cancer treatment; fungi

• Publisher: Fundacja PSC
• Journal: Alcumena. Pismo Interdyscyplinarne
• Year: 2023
• Issue: 2(14)
• Pages: 101-112

Dates

published

2023

Contributors

author

Barbara Siewert

• Gdański Uniwersytet Medyczny

• https://orcid.org/0009000779990826

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Identifiers

DOI

10.34813/psc.2.2023.7

Biblioteka Nauki

32388082