Wykorzystanie potencjału mikrobiomu środowiskowego w kryminalistyce

• Authors: Agata Jagiełło , Anna Woźniak , Błażej Szczerba , Rafał Płoski , Małgorzata Rydzanicz , Agnieszka Pollak , Alina Frolova , Michał Kowalski , Paweł Łabaj , Andrzej Ossowski , Wojciech Branicki , Kinga Herda , Kamila Marszałek , Renata Zbieć-Piekarska

Title variants

EN

Harnessing the potential of the environmental microbiome in forensic science

Abstracts

EN

A scientific consortium led by the Central Forensic Laboratory of the Police has undertaken to develop a method for DNA analysis of the soil microbiome to be used in forensic investigations. The aim of the project entitled Soil Microbiome Analysis Forensic Tool – SMAFT (http://smaft.eu/), financed by the National Center for Research and Development (DOB-BIO10/03/01/2019), is to develop a new tool that enables the association of a trace in the form of a soil sample with a specific geographical location. The first part of the paper introduces the concept of the microbiome and presents the possibilities of using microbiome DNA analysis in forensic science. In the second part, the stages of the SMAFT project are described in detail, beginning from the collection of soil samples from different sites in Poland across all seasons and isolation of microbiome DNA through massively parallel sequencing (MPS) technology-based analysis of isolates and the development of a genetic test containing a set of metagenomic markers allowing for effective individualization of soil samples, up to the creation of an IT system enabling analysis and interpretation of the obtained results, which includes a database of soil microbiome DNA profiles from various locations in Poland.

PL

Konsorcjum naukowe pod przewodnictwem Centralnego Laboratorium Kryminalistycznego Policji podjęło się opracowania metody analizy DNA mikrobiomu gleby, która znajdzie zastosowanie w badaniach kryminalistycznych. Celem projektu o akronimie SMAFT (Soil Microbiome Analysis Forensic Tool, http://smaft.eu/), finansowanego przez Narodowe Centrum Badań i Rozwoju (DOB-BIO10/03/01/2019), jest stworzenie nowego narzędzia umożliwiającego powiązanie śladu w postaci próbki gleby z określoną lokalizacją geograficzną. W pierwszej części artykułu przybliżono pojęcie mikrobiomu oraz przedstawiono możliwości wykorzystania analiz DNA mikrobiomu w kryminalistyce. W jego drugiej części szczegółowo opisano etapy realizowanego projektu, począwszy od zbierania próbek gleby z różnych miejsc Polski w czterech porach roku i izolacji z nich DNA mikrobiomów, poprzez oparte na technologii MPS (ang. Massively Parallel Sequencing) sekwencjonowanie izolatów oraz opracowanie testu genetycznego zawierającego zestaw markerów metagenomicznych pozwalających na skuteczną indywidualizację próbek gleby, aż po stworzenie systemu informatycznego umożliwiającego analizę i interpretację otrzymanych wyników, który obejmuje bazę danych profili DNA mikrobiomów gleb pochodzących z różnych miejsc Polski.

Keywords

PL

mikrobiom; gleba; kryminalistyka; metagenomika; analizy metataksonomiczne; sekwencjonowanie; MPS; bioinformatyka; projekt NCBiR

EN

microbiome; soil; forensics; metagenomics; metataxonomic analysis; MPS sequencing; bioinformatics; NCBiR project

• Publisher: Centralne Laboratorium Kryminalistyczne Policji
• Journal: Problemy Kryminalistyki
• Year: 2021
• Issue: 312

Dates

published

2021

Contributors

author

Agata Jagiełło

• Centralne Laboratorium Kryminalistyczne Policji

author

Anna Woźniak

• Centralne Laboratorium Kryminalistyczne Policji

author

Błażej Szczerba

• Ardigen SA

author

Rafał Płoski

• Warszawski Uniwersytet Medyczny

author

Małgorzata Rydzanicz

• Warszawski Uniwersytet Medyczny

author

Agnieszka Pollak

• Warszawski Uniwersytet Medyczny

author

Alina Frolova

• Instytut Biologii Molekularnej i Genetyki Narodowej Akademii Nauk Ukrainy
• Uniwersytet Jagielloński

author

Michał Kowalski

• Uniwersytet Jagielloński

author

Paweł Łabaj

• Uniwersytet Jagielloński

author

Andrzej Ossowski

• Pomorski Uniwersytet Medyczny w Szczecinie

author

Wojciech Branicki

• Centralne Laboratorium Kryminalistyczne Policji
• Uniwersytet Jagielloński

author

Kinga Herda

• Uniwersytet Jagielloński

author

Kamila Marszałek

• Uniwersytet Jagielloński

author

Renata Zbieć-Piekarska

• Centralne Laboratorium Kryminalistyczne Policji

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Identifiers

DOI

10.34836/pk.2021.312.3

Biblioteka Nauki

23050939