The expression of apoptosis-related proteins in patients with ulcerative colitis

• Authors: K. Niewiarowska , A. Lebelt , M. Gryko , A. Pryczynicz , W. Famulski , J. Czyżewska , A. Kemona , V. Piekarska-Dymicka , D. Jagodzińska , K. Guzińska-Ustymowicz
• Languages of publication: EN

Abstracts

Purpose: Recent literature data indicate a key role of apoptosis in the pathogenesis of inflammatory bowel disease. The aim of the study was to evaluate the expression of Bax, Bid, Bcl-2 and Bcl-xl in non-dysplastic and dysplastic epithelium in inflamated mucosa of patients with ulcerative colitis. Methods: The study consists of 18 patients with diagnosed ulcerative colitis. The expression of proteins was determined immunohistochemically. Results: Lack of Bax expression in normal epithelium of the inflamed intestinal mucosa (94.4%) and a weak expression of this protein were found in dysplastic glandular cells (67%). The Bax expression of dysplastic epithelium correlates with reduced severity of chronic inflammation (p<0.005). Bid expression in non-dysplastic glands was found in 67% of cases vs. 16% in dysplastic epithelium that was associated with the occurrence of epithelial erosions or ulcers (p<0.05). Moderate cytoplasmic expression of Bcl-xl was noted in 27.7% of patients in normal epithelium and in 66.1% within dysplastic lesions. Bcl-xl expression in dysplastic glandular cells correlated with the presence of neutrophils in the lamina propria (p <0.05). Conclusions: The immunohistochemical expressions of Bax, Bcl-2 and Bcl-xl increase and Bid protein expression decreases in dysplastic glandular tubes as compared to non-dysplastic intestinal epithelium in inflamed mucosa, which may suggest an imbalance of controlled cell death in ulcerative colitis.

Keywords

EN

Ulcerative colitis; Bcl-xl; Bax; Bid; Bcl-2

• Publisher: Uniwersytet Medyczny w Białymstoku
• Journal: Progress in Health Sciences
• Year: 2014
• Volume: 4
• Issue: 1
• Pages: 75-82

Contributors

author

K. Niewiarowska

author

A. Lebelt

author

M. Gryko

author

A. Pryczynicz

author

W. Famulski

author

J. Czyżewska

author

A. Kemona

author

V. Piekarska-Dymicka

author

D. Jagodzińska

author

K. Guzińska-Ustymowicz

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