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The paper presents the results of research conducted on cells isolated from the epidermal layer of the skin taken from the ear of domestic swine (Sus scrofa). It was checked whether the increase in the concentration of carbon monoxide (CO) in cell culture, cause changes in their viability, and will affect the changes in the production of nitric oxide. For evaluation of the viability of cells, the MTT assay was used. The concentration of a nitric oxide metabolites (NO3/NO2), in the cell supernatants was measured using test-Measure iT High-Sensitivity Nitrite Assay Kit.
EN
The paper presents the results of viability of breast cancer cells under the influence of electromagnetic field. MCF-7 cell line was subjected to an electromagnetic field with a frequency of 5 Hz, 60 Hz and 120 Hz and an MTT assay was performed immediately after the influence of the field and after 24 hours. No statistical difference was demonstrated in cell viability immediately after exposure to EMF, and there are demonstrated differences in the case of field frequencies of 5 Hz and 120 Hz within 24 hours after exposure.
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