Results found: 1

Search results

Search:
in the keywords: MGMT

Sort By:

Limit search:

Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

100%

Wu F., Liu J., Qiu Y.-L., Wang W., Zhu S.-M., Sun P., Miao W.-B., Li Y.-L., Brandt-Rauf P. W., Xia Z.-L.

International Journal of Occupational Medicine and Environmental Health

2013

vol. 26

issue 1

173-182

Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.

Refine search results

1 International Journal of Occupational Medicine and Environmental Health

1 Brandt-Rauf P. W.

1 Li Y.-L.

1 Liu J.

1 Miao W.-B.

1 Qiu Y.-L.

1 Sun P.

1 Wang W.

1 Wu F.

1 Xia Z.-L.

1 Zhu S.-M.

1 2013

Search results

Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers