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EN
This paper dwells on the uvological characteristics of cultivar Vitis vinifera L. ‘Red Aladasturi’ grape raw materials growing in the viticulture and winemaking zone of Imereti (Georgia), as well as biologically active compounds and antioxidant activity of hydrophilic extracts and liquid concentrates of its solid matters (stone and skin). Research also covered hydrophilic extracts of grape skin and stone thickened by the vacuum of ‘Red Aladasturi’ grapes raw materials, as well as the concentrates produced from their composition. For research, there were used gravimetric, extractive, spectral and chromatographic methods. We processed samples of grapes raw materials according to the following pattern: identifying qualitative indicators of grapes raw materials; passing grapes raw materials through the DMCSI-type grape clustercomb divider; pressing-out the combless must in a basket press and separation of juice; vacuum sublimation drying of juiceless sweet pomace with an initial moisture content of 45–65% to a final moisture content of 9–10%; separation of the ‘Red Aladasturi’ cultivar’s skin and stone dried to the moisture content of 9–10%, using tea sorting machine designed by G. Lominadze; crushing separately the skin and stone in a micro-mill (TP2 Hammer Mill) until the fraction of 50–100 µm. we have blended the obtained grape-stone ethanol and fluid extracts containing 74–75% of solid maters at an equal ratio (1:1:1) and assessed biologically active compounds and antioxidant activity in this composition. It has been established that the bio-flavanoid liquid concentrate ‘Red Aladasturi’ is strong antioxidant  (55.31–57.45%), and one tablespoon or 8–9 ml of it contains 110–127 mg of flavanoids, which is 105–110% of a full day of rations per person per day.
EN
Introduction: Plant extracts rich in phenolic compounds may modify the stress level. The aim of this project is to investigate whether freeze-dried rosehips (Rosa rugosa), taken orally by students, affect cortisol concentration before and after a highly stressful exam. Materials and methods: The double-blind study included healthy volunteers recruited from medical and health sciences students. Participants in the study group took capsules containing 400 mg of freeze-dried ground rosehips for five consecutive days preceding the exam (stressor). At that time, the control group was taking a placebo (120 mg of glucose). Participants completed a questionnaire on their gender, lifestyle, medical history, physical activity, diet, stimulants, medications and dietary supplements. Saliva samples for determining the level of cortisol in saliva were taken three times: in the morning before the beginning of the experimental phase, in the morning before the stressor, and one hour after the stressor subsided. The level of cortisol in saliva was determined using ELISA. Results: It was found that taking freeze-dried rosehips for five days significantly reduced the level of cortisol in students after the exam compared to the placebo group. There were no differences in the level of cortisol before the exam, regardless of the preparation taken. No other analyzed factor influenced the concentration of the stress hormone in students. Conclusions: Freeze-dried rosehips can help reduce the effects of stress.
PL
Wprowadzenie: Ekstrakty roślinne bogate w polifenole mogą modyfikować poziom stresu. Celem projektu było zbadanie, czy liofilizowane owoce róży (Rosa rugosa) przyjmowane doustnie przez studentów wpływają na poziom kortyzolu przed i po silnie stresującym egzaminie. Materiał i metody: Badanie o charakterze podwójnie ślepej próby objęło zdrowych ochotników rekrutowanych pośród studentów kierunków medycznych. Uczestnicy w grupie badanej przyjmowali kapsułki zawierające po 400 mg liofilizowanych mielonych owoców róży przez pięć kolejnych dni poprzedzających egzamin (stresor). W tym czasie grupa kontrolna przyjmowała placebo. Uczestnicy wypełnili ankietę dotyczącą ich płci, stylu życia, historii chorób, aktywności fizycznej, stosowanej diety, używek, leków i suplementów diety. Próbki śliny do oznaczenia poziomu kortyzolu zostały pobrane trzykrotnie: rano przed rozpoczęciem fazy eksperymentalnej, rano przed stresorem oraz godzinę po ustąpieniu stresora. Poziom kortyzolu w ślinie oznaczono metodą immunoenzymatyczną. Wyniki: Stwierdzono, że przyjmowanie liofilizowanych owoców róży przez pięć dni istotnie zmniejsza poziom kortyzolu u studentów po egzaminie w porównaniu z grupą przyjmującą placebo. Nie wykazano różnic w poziomie kortyzolu przed egzaminem niezależnie od przyjmowanego preparatu. Żaden inny analizowany czynnik nie wpływał na stężenie hormonu stresu u studentów. Wnioski: Liofilizowane owoce róży mogą pomagać w osłabieniu skutków stresu.
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