Dodoru Market is popular for its weekly Market day in Kebbi State, Nigeria. People converge at the market every Monday from various places to buy or sell. Survey of ticks on camels and cattle brought to Dodoru Market for sale was conducted. The aim was to evaluate the prevalence of camel and cattle ticks. A total of 90 animals (40 camels and 50 cattle) were examined. Five tick species were identified and prevalence was evaluated. Results revealed that 32 of the camels were infected with the prevalence of 35.6% and cattle were also infested with 44.4 % prevalence. Boophilus decoloratus has the highest prevalence of 29.1 % in both camels and cattle. Thigh and abdomen were more susceptible to ticks species with the prevalence of 37.7 %. The survey also shows that cattle were more infested than camels and thigh/abdomen was observed to be the most preferred locations for ticks in both camels and cattle.
Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya province, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.