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EN
Background The objective of the study has been to evaluate the pathogenic bacteria contamination of touch surfaces in hospital wards. Material and Methods Samples were taken from frequently touched surfaces in the hospital environment in 13 units of various types. Culturing was carried out on solid blood agar and in growth broth (tryptic soy broth – TSB). Species identification was performed using the analytical profile index (API) biochemical testing and confirmed with matrix assisted laser desorption ionization time-flight mass spectrometry (MALDI-TOF-MS) system. Results The total of 161 samples were taken for the study. Fifty-two of them, after 24 h of culture on a solid medium, demonstrated bacterial growth and further 60 samples had growth after prior multiplication in TSB. Overall, 69.6% of samples exhibited growth of 19 bacterial species. Pathogenic species – representing indicator organisms of efficiency of hospital cleaning – was demonstrated by 21.4% of samples. Among them Acinetobacter spp., Enterocococci spp. and Staphylococcus aureus were identified. Coagulase-negative staphylococci (CNS) were predominant. The proportion of various groups of bacteria significantly varied in respective hospitals, and in various types of wards. Disturbing observation is a large proportion of resistance of isolated CNS strains as a potential reservoir of resistance genes. Conclusions The results show that touch surfaces in hospital units are contaminated by both potentially pathogenic and pathogenic bacterial species. In connection with the reported, also in Poland, frequent omission or incorrect execution of hand hygiene by hospital staff, and probably patients, touch surfaces still constitute important reservoir of pathogenic bacteria. Improving hand hygiene compliance of health-care workers with recommendations is necessary for increasing biological safety of hospital environment. Med Pr 2017;68(3):459–467
EN
This paper attempts to determine the role of the hospital environment in the spread of Clostridioides/Clostridium (C.) difficile infections based on a review of studies published in the medical literature and in the light of the authors’ own experiences. Clostridioides/Clostridium difficile has recently attracted more and more attention, not only as an etiological factor of pseudomembranous intestinal inflammation and antibiotic associated diarrhea, but also as an etiologic factor of healthcare-associated infections (HAI) because of the possibility to survive in the hospital environment for a long time. This is caused by the production of spores, whose eradication options are limited. Clostridioides/Clostridium difficile spores are present not only on bedding of infected patients and their other belongings, but also on medical equipment and the hands of medical personnel, constituting a potential source of infection for other patients and some of the staff. The introduction of appropriate procedures for hand hygiene as well as for cleaning and disinfection of hospital surfaces makes it possible to reduce the number of spores and/or eradicate them. These procedures must be strictly followed to reduce the occurrence of spores in the hospital environment and to prevent further spread of C. difficile infections (CDI). Monitoring the presence of the C. difficile spores in a hospital environment using appropriate media (C diff Banana BrothTM) provides additional opportunities for culturing of C. difficile strains and determining ribotypes, especially hyperepidemic ones, which is extremely important from an epidemiological point of view. Med Pr. 2019;70(6):739–45
PL
W artykule podjęto próbę określenia roli środowiska szpitalnego w szerzeniu się zakażeń Clostridioides/Clostridium (C.) difficile na podstawie przeglądu wyników badań opublikowanych w literaturze medycznej i własnych doświadczeń. Clostridium difficile ostatnio przyciąga coraz więcej uwagi, nie tylko jako czynnik etiologiczny rzekomobłoniastego zapalenia jelit i biegunek poantybiotykowych, ale także – ze względu na zdolność przetrwania w środowisku szpitalnym przez długi czas – jako przyczyna zakażeń związanych z opieką zdrowotną. Jest to spowodowane wytwarzaniem przez C. difficile przetrwalników – spor, których możliwości zwalczania są dość ograniczone. Spory C. difficile obecne są nie tylko na pościeli i innych przedmiotach należących do zakażonych pacjentów. Bytują także na sprzęcie medycznym i dłoniach personelu, które stanowią źródło zakażenia zarówno dla innych pacjentów, jak i dla części personelu. Wprowadzenie odpowiednich procedur higieny/mycia rąk oraz sprzątania i dezynfekcji powierzchni szpitalnych umożliwia zmniejszenie liczby spor i/lub ich eradykację. Procedur tych należy skrupulatnie przestrzegać w celu ograniczenia występowania spor w środowisku szpitalnym i zapobiegania dalszemu szerzeniu się zakażeń C. difficile (Clostridium difficile infection – CDI). Monitorowanie obecności spor C. difficile w środowisku szpitalnym z zastosowaniem odpowiednich podłóż (C diff Banana BrothTM) daje dodatkowe możliwości wyhodowania szczepów C. difficile i określenia rybotypów, zwłaszcza hiperepidemicznych, co jest niezmiernie ważne z punktu widzenia epidemiologicznego. Med. Pr. 2019;70(6):739–745
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