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1

Cadmium acts as a silent killer of liver by inducing oxidative stress and hepatocellular injury and a possible amelioration by vitamin B12 and folic acid in rat model

100%

Banerjee A., Nandi P., Bhattacharya C., Kabir Z., Mukherjee S., Maji B.

Progress in Health Sciences

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2019

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vol. 1

105-117

EN

Purpose: To investigate the involvement of oxidative stress in Cadmium (Cd) induced alteration in the functional status of the liver. And to assess the efficacy of folic acid and vitamin B12 in preventing Cd-induced damage in the same. Materials and methods: The experiment was carried out for four weeks. For the experiment, 25 healthy male adult Wistar albino rats were randomly selected and were divided into five equal groups and treated as control, treated with Cd, supplemented with vitamin B12 and folic acid and in the combination of these two. After 28 days the liver function enzymes and oxidative stress parameters were measured. Results: Cd is the silent killer of the hepatic system through the induction of oxidative stress in male rats. From this investigation, it is evident that the folic acid+vitamin B12 possess significant hepatoprotective and antioxidant activity against Cd-induced hepatotoxicity in the rat model. In addition, results revealed that the folic acid alone and or in combination with vitamin B12 blunted the hepatotoxic effect significantly. Conclusions: Based on results obtained, it can be concluded that folic acid and vitamin B12 offer a protective effect in Cd-induced oxidative stress associated with hepatocellular injury. Folic acid and vitamin B12 can be considered as a potent natural antioxidant which has the capacity to provide protection against Cd-induced oxidative stress in the liver in rats. However, to elucidate the exact mechanism of this modulatory effect and to examine its potential therapeutic effects further studies are essential.

2

A proposal for calculating the no-observed-adverse-effect level (NOAEL) for organic compounds responsible for liver toxicity based on their physicochemical properties

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Jakubowski M., Czerczak S.

International Journal of Occupational Medicine and Environmental Health

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2014

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vol. 27

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issue 4

627-640

EN

Objectives: Both environmental and occupational exposure limits are based on the no-observed-adverse-effect level (NOAEL), lowest-observed-adverse-effect level (LOAEL) or benchmark dose (BMD) deriving from epidemiological and experimental studies. The aim of this study is to investigate to what extent the NOAEL values for organic compounds responsible for liver toxicity calculated based on their physicochemical properties could be used for calculating occupational exposure limits. Material and Methods: The distribution coefficients from air to the liver (log $\text{K}_\text{liver}$) were calculated according to the Abraham solvation equation. NOAEL and LOAEL values for early effects in the liver were obtained from the literature data. The descriptors for Abraham's equation were found for 59 compounds, which were divided into 2 groups: "non-reactive" (alcohols, ketones, esters, ethers, aromatic and aliphatic hydrocarbons, amides) and "possibly reactive" (aldehydes, allyl compounds, amines, benzyl halides, halogenated hydrocarbons, acrylates). Results: The correlation coefficients between log-log K and log NOAEL for non-reactive and reactive compounds amounted to r = -0.8123 and r = -0.8045, respectively, and were statistically significant. It appears that the Abraham equation could be used to predict the NOAEL values for compounds lacking information concerning their liver toxicity. Conclusions: In view of the tendency to limit animal testing procedures, the method proposed in this paper can improve the practice of setting exposure guidelines for the unstudied compounds.

3

Czynniki ryzyka włóknienia wątroby u osób zakażonych hcv - doniesienia wstępne

75%

Dzieńdziora-Frelich I.

Puls Uczelni

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2013

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issue 1

7-9

PL

Wstęp: HCV stanowi poważny problem w praktyce medycznej. Większość powikłań w przebiegu chorób wątroby jest konsekwencją zaawansowanego włóknienia prowadzącego nawet do marskości wątroby. Cel pracy: Celem niniejszych badań jest analiza wybranych czynników ryzyka rozwoju włóknienia wątroby u osób z zakażeniem hcv. Materiał i metody: Materiał badawczy stanowiła grupa pacjentów mieszkających na terenie województwa śląskiego. Badaną grupę stanowiły 102 osoby w wieku 13-70 lat. chorzy diagnozowani byli na oddziałach chorób zakaźnych i chorób wewnętrznych z podejrzeniem hcv. Ostateczne rozpoznanie ustalono na podstawie wywiadu z pacjentami oraz oceny histopatologicznej biopsji wątroby. Wyniki: W niniejszych badaniach udział wzięło 66 mężczyzn i 36 kobiet. Przedział wiekowy 13-23 lat stanowi 7,8%, 13, 7% to populacja w wieku 24-34 lat, 32, 4% to badani w wieku 35-45 lat, 27, 5% - pacjenci w przedziale wiekowym 46-56 lat, 16, 7% to populacja w wieku 57-67 lat, 1,9% badanych stanowiła grupa powyżej 67 roku życia. nadwaga występowała u 55,9% badanych, 76, 5% populacji to osoby palące. Wnioski: Na podstawie analizy badań stwierdza się, że wirus hcv z postępującym włóknieniem najczęściej występuje w przedziale wiekowym 35-45 lat, częściej wśród mężczyzn niż wśród kobiet. większość badanych to osoby palące papierosy oraz osoby z nadwagą.

EN

Introduction: Hcv constitutes a serious problem in medical practice. Most complications in the course of the disease derive from advanced fibrosis leading even to the liver cirrhosis. Aim of the research: The aim of the research is the analysis of some selected risk factors of the development of fibrosis in patients with hcv and the frequency of the virus infections in The Silesia Region. Material and methods: The material was collected from the patients living in The Region of Silesia. The population for the research consisted of 102 patients at the age of 13 – 70. The patients were diagnosed as sufering from hcv by The Infectious and Internal Diseases Departments. The final diagnosis was made after interviewing the patients and the examinations of histopathological liver biopsy. The results: There were 66 males and 36 females participating in the research. Patients aged 13-23 ( 13) constituted 7,8 per cent, 24-34 (32) 7 per cent, 35-45 (27) 4 per cent, 46-56 (16) 5 per cent, 57-67 7 per cent and 2 per cent of patients over 67. 55,9 per cent of the patients were overweight and 76,5 per cent smokers. Conclusions: Taking into account the research analysis, it might be concluded that the hcv virus accompanied by liver fibrosis is found most often in the group of patients aged 35-45 and more often in males than in females. Most research patients are overweight and smoke cigarettes.

4

Role of sulfide anion in the development of chronic alcoholic hepatitis under the conditions of modulation of adenosine monophosphate kinase – a correlational study

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Mykytenko A., Akimov O., Shevchenko O., Neporada K.

European Journal of Clinical and Experimental Medicine

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2023

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issue 3

567-575

EN

Introduction and aim. Hydrogen sulfide (H2S) has attracted the attention of researchers as a novel signaling molecule that affects vascular metabolism, immune function, stress and inflammation. It plays an important role in pathophysiological disorders under the conditions of the development of obesity, diabetes, non-alcoholic fatty liver disease and cardiovascular diseases. The purpose of this work is to establish correlation ratios of H2S concentration with markers of oxidative-nitrosative stress and extracellular matrix metabolism of the liver during chronic alcoholic hepatitis modeling and AMPK modulation by phenformin and doxorubicin. Material and methods. The experiments were performed on 36 white, sexually mature male Wistar rats, weighing 180-220 g. Alcoholic hepatitis was modelled by alcohol administration, on the background of alcoholic hepatitis animals received phenformin orally at a dose of 10 mg/kg or doxorubicin at a dose of 1.25 mg/kg intraperitoneally. Statistical processing of the results of biochemical studies was carried out using the non-parametric method of Spearman to determine correlations. Results. H2S during alcoholic hepatitis inversely proportionally strongly correlates with the concentration of nitrites, oxyproline and arginase activity. Phenformin administration during alcoholic hepatitis leads to formation of inversely proportionally strongly correlation of H2S with the production of superoxide anion radical, the concentration of malondialdehyde, activities of constitutive NO-synthases, nitrite reductases, nitrate reductases, and arginase. Doxorubicin administration during alcoholic hepatitis leads to formation of directly proportional strongly correlation of H2S with the activity of constitutive NO-synthases, nitrite reductases, nitrate reductases. Conclusion. Administration of phenformin or doxorubicin expands correlations between H2S and indicators of oxidative-nitrosative stress.

5

Rola argininy w organizmie człowieka

63%

Zdrojewicz Z., Winiarski J., Popowicz E., Szyca M.

Zeszyty Naukowe Państwowej Wyższej Szkoły Zawodowej im. Witelona w Legnicy

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2019

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issue 1(30)

PL

Celem niniejszego opracowania jest przedstawienie roli argininy w organizmie człowieka. Arginina jest aminokwasem względnie egzogennym, co oznacza, że pomimo tego, iż jest produkowana w ludzkim ciele, to dostarczanie jej wraz z pożywieniem jest równie ważne dla zachowania homeostazy organizmu. Główne źródło argininy w diecie stanowią mięso i owoce morza. Aminokwas ten jest syntezowany w czasie cyklu mocznikowego w wątrobie, a także w nerkach. Bierze udział między innymi w wytwarzaniu tlenku azotu, który jest czynnikiem rozszerzającym naczynia. Liczne prace wskazują również na skuteczność suplementacji argininą w zaburzeniach erekcji. Udział argininy w tej reakcji przyczynił się do zainteresowania wielu badaczy. Dowiedziono, że oprócz powszechnie znanego zastosowania argininy jako leku wspomagającego wątrobę w usuwaniu z organizmu amoniaku, odpowiednia suplementacja tego aminokwasu ma pozytywny wpływ na poprawę wartości ciśnienia tętniczego indukowanego ciążą, nadciśnienia płucnego oraz wyrównanie niewydolności nerek, cukrzycy, jaskry, gojenia się ran i profilaktykę udarów mózgu. Uważa się, że stężenie argininy wpływa na rozwój chorób neurodegeneracyjnych postępujących z wiekiem. Aminokwas ten jest też środkiem chętnie stosowanym przez sportowców w celu poprawienia wydolności organizmu. Jednak przeprowadzone do tej pory badania nie potwierdzają jego pozytywnego wpływu w tym zakresie.

EN

The aim of this study is to show the role of arginine in the human body. Arginine is a relatively exogenous amino acid, which means that, although it is produced in the hu- Zygmunt Zdrojewicz, Jacek Winiarski, Ewa Popowicz, Marta Szyca… 171 man body, supplying it with food is equally important for maintaining homeostasis of the body. The main source of arginine in the diet is meat and seafood. This amino acid is synthesized during the urea cycle in the liver as well as in the kidneys. It is involved, among other processes, in the production of nitric oxide, which is a vasodilator. Numerous studies also indicate the efficacy of arginine supplementation in erectile dysfunction. The contribution of arginine in this reaction has generated interest from many researchers. It has been proven that in addition to the well-known use of arginine as a drug contributing to the removal of ammonia from the body, adequate supplementation of this amino acid has a positive effect on the improvement of pregnancy-induced hypertension, pulmonary hypertension, chronic renal failure, diabetes, glaucoma, wound healing and stroke prevention. It is believed that the concentration of arginine affects the development of neurodegenerative diseases progressing with age. This amino acid is also willingly used by athletes to improve the efficiency of the body. However, the studies carried out so far do not confirm its positive impact in this respect

6

Attenuating effects of caffeic acid phenethyl ester with intralipid on hepatotoxicity of chlorpyrifos in the case of rats

51%

Dokuyucu R., Bilgili A., Hanedan B., Dogan H., Dokuyucu A., Celik M. M.

Medycyna Pracy

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2016

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vol. 67

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issue 6

743-749

PL

Wstęp Chloropiryfos (CPF), środek owadobójczy szeroko stosowany w rolnictwie, może powodować zatrucia u ludzi. Z tego powodu prowadzi się wiele badań dotyczących możliwości leczenia zatrucia chloropiryfosem. Celem pracy była ocena wpływu estru fenetylowego kwasu kawowego (caffeic acid phenethyl ester – CAPE) i intralipidu (IL) na uszkodzenia wątroby u szczurów wywołane przez chloropiryfos. Materiał i metody Szczurom podawano CPF (10 mg/kg masy ciała (mc.), doustnie), CAPE (10 μmol/kg mc., dootrzewnowo), IL (18,6 ml/kg mc., doustnie), CPF+CAPE, CPF+IL oraz CPF+CAPE+IL. Zmierzono całkowitą zdolność utleniającą (total oxidant capacity – TOC) i całkowitą zdolność przeciwutleniającą (total antioxidant capacity – TAC) osocza krwi i obliczono wskaźnik stresu oksydacyjnego (oxidative stress index – OSI). U zwierząt wykonano także badanie histopatologiczne i barwienie immunohistochemiczne tkanek wątroby. Wyniki Chloropiryfos istotnie zmniejszał u badanych szczurów TAC osocza, a zwiększał apoptozę, TOC i OSI. Natomiast CAPE i CAPE+IL istotnie zmniejszały OSI osocza i apoptozę, a zwiększały TAC osocza u szczurów z uszkodzeniami wątroby wywołanymi przez chloropiryfos. Wnioski Badanie wykazało, że CAPE i CAPE+IL poprzez zmniejszenie stresu oksydacyjnego i apoptozy redukują u szczurów uszkodzenia wątroby wywołane przez chloropiryfos. Med. Pr. 2016;67(6):743–749

EN

Background Chlorpyrifos (CPF), insecticide widely used in agriculture, may cause poisonings in the case of humans. As a result, there is a large amount of treatment research underway to focus on the possibility of chlorpyrifos induced poisonings. The aim of this study has been to evaluate the effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity induced by chlorpyrifos in the case of rats. Material and Methods The rats in this study were treated with CPF (10 mg/kg body weight (b.w.), orally), CAPE (10 μmol/kg b.w., intraperitoneally), IL (18.6 ml/kg b.w., orally), CPF+CAPE, CPF+IL, and CPF+CAPE+IL. The plasma total oxidant capacity (TOC), total antioxidant capacity (TAC) were measured and the oxidative stress index (OSI) was calculated. Liver histopathology and immunohistochemical staining were performed. Results Chlorpyrifos statistically significantly decreased the TAC levels in the rats’ plasma and increased the apoptosis and the TOC and OSI levels. In the chlorpyrifos induced liver injury, CAPE and CAPE+IL significantly decreased the plasma OSI levels and the apoptosis, and significantly increased the plasma TAC levels. Conclusions This study revealed that CAPE and CAPE+IL attenuate chlorpyrifos induced liver injuries by decreasing oxidative stress and apoptosis. Med Pr 2016;67(6):743–749

Refine search results

Cadmium acts as a silent killer of liver by inducing oxidative stress and hepatocellular injury and a possible amelioration by vitamin B12 and folic acid in rat model

A proposal for calculating the no-observed-adverse-effect level (NOAEL) for organic compounds responsible for liver toxicity based on their physicochemical properties

Czynniki ryzyka włóknienia wątroby u osób zakażonych hcv - doniesienia wstępne

Role of sulfide anion in the development of chronic alcoholic hepatitis under the conditions of modulation of adenosine monophosphate kinase – a correlational study

Rola argininy w organizmie człowieka

Attenuating effects of caffeic acid phenethyl ester with intralipid on hepatotoxicity of chlorpyrifos in the case of rats