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Dietary supplementation with algae and polyphenols in rabbit male: Effects on semen quality traits

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Vizzarri F., Palazzo M. M., Casamassima D., Corino C., Chiapparini S., Ondruska L., Knizatova N., Massanyi M., Tirpak F., Massanyi P.
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
|
2018
EN
In recent years, many studies have been focused on natural substances that can affect health of animals. A mix of different extracts was used as dietary supplement and it consists exclusively of natural products. Its main components are polyphenols from terrestrial and marine origins and plant polysaccharides. The effect of this supplement on reproduction has not been reviewed in the past what is a reason why its effect on the reproduction potential of male rabbits was tested. The aim of the present study is to determine effects of natural mix during 120-days long in vivo experiment on selected reproductive traits of male rabbits. Natural mix was supplemented in two different concentrations (T1 – 0.3% and T2 – 0.6%) with the basal ingredients of the conventional rabbit feed in pellet form. In our experiments, emphasis was placed on both the spermatozoa concentration and its motility parameters as well as on the properties of seminal plasma and antioxidant activity. The dietary supplementation with the natural extracts mix positively altered the quality traits of rabbit spermatozoa, but these changes were statistically not significant. In experimental T1 group a significant increase of GPx and FRAP content, both regarding the antioxidant markers profile in seminal plasma was recorded. We can conclude that the supplementation of 0.3% of natural mix did not significantly negatively affect any of the studied reproductive parameters of male rabbits, but some improvement in several antioxidant parameters was found.
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Different MACS sorting strategies for the enrichment of Lin– (CD34+ CD45–) hematopoietic progenitor cells: preliminary study

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Vašíček J., Baláži A., Parkányi V., Bauer M.
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
|
2018
EN
Magnetic-activated cell sorting (MACS) has become a standard method for the isolation of hematopoietic stem/progenitor cells (HSC/HPC) in human or mouse model using CD34 antibodies. However, at the present there is no useable CD34 antibody that could be successfully used for the selection of rabbit HSC/HPC. Therefore, the aim of this preliminary study was to remove all mature cells (CD45+) from the heterogeneous mixture of rabbit peripheral blood and bone marrow mononuclear cells (PBMCs and BMMCs) in order to enrich these cell populations for the CD34+ cells. Briefly, cells were incubated with a CD45 antibody and proper magnetic microbeads. Three different MACS sorting strategies were used in the experiment that differed mainly in the sample loading rate and the number of used magnetic columns. Control (unsorted) and sorted cells were assessed for the sorting efficiency (% of double positive cells for CD45 and Labelling Check Reagent – LCR) by flow cytometry and for the relative expression of CD34 antigen by qPCR. According to flow cytometry, Depl025 mode showed the best sorting efficiency in terms of the lowest percentages of CD45+LCR+ cells for rabbit PBMCs as well as BMMCs. qPCR analysis confirmed this mode as the best in terms of the relative CD34 expression for rabbit PBMCs. However, higher relative expression of CD34 in BMMCs was obtained by other mode – Posselds. In conclusion, this study demonstrates a possible enrichment of rabbit (CD34+) HSC/HPC by the magnetic depletion of mature hematopoietic (CD45+) cells.
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