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EN
The aim of this study was to investigate the influence of copper and vanadium ions on photosynthetic activity of carrot (Daucus carota L. subsp. sativus (Hoffm.) Schübl. & G. Martens) and winter wheat (Triticum aestivum L.). Measurements of the total chlorophyll content were performed – the SPAD chlorometer and the basic chlorophyll a fluorescence parameters were determined using the FMS-1 fluorometer – Hansatech. The studies used aqueous solutions of copper salt (CuSO4) and vanadium (H4NO3V), with molar concentrations: 0.6 mM, and 3 mM. The control group consisted of plants watered with distilled water. Both in carrots and in wheat, together with an increase in the concentration of heavy metal ions, a decrease in the content of chlorophyll was observed and significant changes in the activity of the photosystem were demonstrated II. Measurements of chlorophyll fluorescence kinetics and only in D. carota subsp. sativus showed a statistically significant effect of 3 mM solutions of copper and vanadium ions on photosynthetic activity. In T. aestivum none of the heavy metal ions induced significant changes in the values of chlorophyll a fluorescence.
EN
In the whole world, researches are conducting on toxic effect of heavy metals on living organisms. The problem with heavy metal occurrence in the environment is not only associated with their toxicity, but also with their ability to accumulate inside living organisms. This study presents the effect of copper and vanadium ions on germination and growth of carrot (Daucus carota L subsp. sativus (Hoffm.) Schübl. & G. Martens.) and winter wheat (Triticum aestivum L.). The experiment was carried out in two independent series with ten repetitions each. The water solutions of copper (CuSO4) and vanadium (H4NO3V) salts with the concentrations: 0.6 mM, 0.3 mM, 3 mM and 6 mM were used. The control groups were objects watered with distilled water. The conducted experiment showed that, the copper and vanadium ions had negative effect on germination and growth of plants. With the increasing of concentration of heavy metal ions an inhibition of seeds germination capacity was observed. The length of D. carota and T. aestivum seedlings in each salt solutions was inhibited, compared to the control group. During the growth phase, the stimulation of wheat leaves growth, only on copper solutions with concentration 3 mM and 6 mM was observed. Changes of a fresh and dry weight, and a water content were observed, depending on the copper and vanadium ions concentration.
EN
Purpose: Data are available that indicate there is an elaborate elemental constitution of petroleum products, with identified elements contained in the many products being additive (e.g. Ca, Zn and P) as well as wear metals (e.g. Ag, Al, Ba, Cd, Cr, Cu, Fe, Mg, Mo, Na, Ni, Pb and Sn). In addition, incessant deliberate exposure of engine oil to both human beings and farm animals for therapeutic reason has been reported. Therefore the objective of this study is to evaluate the levels of heavy metals in serum of engine oil-exposed rats. Materials & Methods: Thirty adult female rats were divided equally into 5 groups. The first and second groups were treated with engine oil by oral route (as contaminant of feed) at dosage levels of 0.5 and 1.0 mL/kg body weight respectively. The third and forth groups received the test agent through the dermal route at dosage levels of 0.5 and 1.0 mL/kg body weight while the fifth group served as the control. The duration of the study was 30 days, after which blood was obtained from each rat, centrifuged and the resultant serum used for the analysis of heavy metals by employing Atomic Absorption Spectrometry (AAS). Data were analyzed using analysis of variance (ANOVA), p≤0.05 was considered significant. Results: Data obtained showed that there were significant differences in the levels of aluminium, silicon, cadmium, lead, arsenic, vanadium, and nickel. Conclusions: These increases suggest that incessant exposure to engine oil may be dangerous and therefore constitute health hazard.
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