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This paper discusses the issues related to the influence of mould fungi on tempera painting on a panel made of wood. Samples of lime-wood were covered with glue and chalk ground, painted with egg tempera of a specific colour and then exposed to mould fungi. After an incubation period, the painting layer was submitted to a computer image analysis. The results of the analyses demonstrated no correlation between the degree of the test fungi growth on the sample and the change in the colour or its shade.
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