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Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

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Wu F., Liu J., Qiu Y.-L., Wang W., Zhu S.-M., Sun P., Miao W.-B., Li Y.-L., Brandt-Rauf P. W., Xia Z.-L.

International Journal of Occupational Medicine and Environmental Health

2013

vol. 26

issue 1

173-182

Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.

Polymorphisms in the p53 pathway genes and micronucleus occurrence in Chinese vinyl chloride-exposed workers

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Li Y., Feng N.-N., Zhang G.-H., Wang Q., Hao Y.-H., Nanzhang Y., Long C., Li Y., Brandt-Rauf P. W., Xia Z.-L.

International Journal of Occupational Medicine and Environmental Health

2013

vol. 26

issue 6

825-836

Objectives: To investigate the association between polymorphisms in the p53 pathway genes and chromosomal damage in vinyl chloride (VC)-exposed workers. Materials and Methods: Cytokinesis block micronucleus test was performed in 310 VC-exposed workers and 149 non-exposed workers to determine chromosomal damage. The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect six SNPs in the p53 pathway genes involved in the cell cycle. Results: There was a highly significant dose-response relationship between VC exposure and chromosomal damage. Individuals carrying the variant genotypes were at higher risk for chromosomal damage compared with their wild type genotype: p53rs1042522, MDM2 Del1518rs3730485, MDM2rs2279744 and GADD45Ars532446. On the other hand, individuals possessing the variant genotype of CDKN2A rs3088440 had significantly decreased risk compared with the corresponding wild-type. Conclusions: Genetic polymorphisms in P53 pathway genes may have an impact on VC-induced chromosomal damage.

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2 International Journal of Occupational Medicine and Environmental Health

2 Brandt-Rauf P. W.

2 Xia Z.-L.

1 Feng N.-N.

1 Hao Y.-H.

1 Li Y.

1 Li Y.-L.

1 Liu J.

1 Long C.

1 Miao W.-B.

1 Nanzhang Y.

1 Qiu Y.-L.

1 Sun P.

1 Wang Q.

1 Wang W.

1 Wu F.

1 Zhang G.-H.

1 Zhu S.-M.

2 2013

Search results

Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers

Polymorphisms in the p53 pathway genes and micronucleus occurrence in Chinese vinyl chloride-exposed workers