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1

Serum and urinary β-glucuronidase in acute alcohol intoxication: a pilot study

100%
Waszkiewicz N., Zalewska-Szajda B., Buras A., Szulc A., Zwierz K., Ładny J., Szajda S.
Progress in Health Sciences
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2012
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vol. 2
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issue 2
52-56
EN
Introduction: Beta-glucuronidase (GLU) is a member of the lysosomal glycosidase family that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of glycosaminoglycans. Increased activities of GLU have been earlier reported in the serum of alcohol-dependent patients after a chronic heavy drinking period but not after acute intoxication (called binge drinking). The accelerating binge drinking pheno-menon is an alarming public health issue that requires better prevention. Purpose: To determine the activity serum and urinary GLU, after an acute, single, and a large dose of alcohol intoxication. Materials and methods: The serum and urine of eight healthy binge drinkers were collected before binge drinking, and 2 and five days after the drinking session. The activity of GLU was determined by the colorimetric method. Results: There was a tendency to decrease in the serum GLU activity two days after acute alcohol intoxication (binge drinking), which was followed by the significant increase in the GLU activity five days after drinking. The urinary activity of GLU was not changed after intoxication. Conclusion: Alcohol-induced imbalance in the serum GLU activity might be associated with alcohol-induced liver hypoxia and subsequent reperfusion, and can be detected even five days after the drinking session.
2

Lyme disease: etiology, pathogenesis, clinical courses, diagnostics and treatment

84%
Wasiluk A., Zalewska-Szajda B., Waszkiewicz N., Kępka A., Szajda D., Wojewódzka-Żeleźniakowicz M., Ładny J., Pancewicz S., Zwierz Z., Zwierz K.
Progress in Health Sciences
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2011
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vol. 1
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issue 2
179-186
EN
Lyme disease (LD) is caused by Borrelia burgdorferi, transferred by infected ticks Ixodus ricinus. LD occurs endemically in Europe, America and Northern regions of Asia. In Poland, LD is the most frequent tick borne disease, which causes serious epidemiological problems. The main health hazard of LD occurs on the forested areas of Podlasie, Maritime province, the West Poland lake district, and Carpathians. The highest incidence 114.0 per 100 000 was registered in Podlaskie province. The infectious cycle of Borrelia burgdorferi includes: nymph moulds to adult, eggs laid by female, eggs hatch to larva, larva feeds on first host, fully fed larva drops to ground, larva moulds to nymph, nymph attaches to and feeds on the second host. LD is a chronic disease attacking many organs, including the skin, heart, brain and joints. LD is divided into three stages based on clinical symptoms: I - limited infection (Erythema migrans and Borrelia lymphoma), II - disseminated infection (numerous erythema migrans, early neuro-borreliosis, joint inflammation, Lyme carditis), III - late borreliosis (chronic atrophic limbs infla-mmation, late neuroborreliosis, chronic joint infla-mmation). At present, the best diagnostic method for LD is a labor- and time consuming two-stage serological method. First-line antibiotics in the treatment of Lyme disease are doxycycline, amoxicillin, cefuroxime axetil, ceftriaxone, cefo-taxime, and penicillin G. The best method of avoiding Borrelia burgdorferi infection is to avoid biting from ticks carrying spirochetes. Early removal of ticks, protects against spirochaetal infection.
3

Serum beta-glucuronidase in colon cancer patients dependent on alcohol and nicotine: preliminary report

84%
Waszkiewicz N., Zalewska-Szajda B., Repka B., Szulc A., Kępka A., Chojnowska S., Dadan J., Ładny J., Zwierz K., Szajda S.
Progress in Health Sciences
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2012
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vol. 2
|
issue 2
64-69
EN
Introduction: Beta-glucuronidase (GLU) is a lysosomal exoglycosidase involved in the catabolism of glycoconjugates. Excessive GLU activity may be a primary factor in the etiology of colon cancer. The stimulation of glycosidases and other degradative enzyme activity has been noted in cancers as well as in alcohol and nicotine addiction. Purpose: To compare the serum GLU activity between alcohol- and nicotine-dependent colon cancer patients and colon cancer patients without a history of alcohol- and nicotine-dependence. Materials and methods: Material was the serum of 22 colon cancer patients, 11 of whom met alcohol and nicotine dependence criteria. The activity of serum GLU (pKat/ml) was determined by the colorimetric method. Carcinoembryonic antigen (CEA) concentration (ng/ml) in the serum was determined by the immunoenzymatic method. Comparisons between groups were made using the Mann-Whitney “U” test. Spearman’s rank correlation coefficient was used to measure the association between two variables. Results: The activity of serum GLU was significantly higher in colon cancer patients with a history of alcohol and nicotine dependence, than in the colon cancer patients without a history of drinking and smoking (p=0.003). There was no significant difference in the CEA concentration between colon cancer patients with and without a history of drinking and smoking. Conclusion: Alcohol and nicotine addiction may increase the serum GLU activity in all cancer patients, as already seen in colon cancer patients. This may potentially be important for the degradation of pericancerous matrix, tumor growth, invasion and metastasis
4

Salivary hexosaminidase B in children with type 1 diabetes

68%
Zalewska-Szajda B., Chojnowska S., Waszkiewicz N., Gościk E., Łebkowska U., Kępka A., Bossowski A., Kuźmiuk A., Mierzyńska K., Szajda S., Ładny J.
Progress in Health Sciences
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2012
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vol. 2
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issue 2
null-140
EN
Introduction: Children type 1 diabetes is accompanied by inflammation and microangiopathy pro-ceeded with increased degradation of tissues. Nacetyl-β-hexosaminidase (HEX) is the most active of exoglycosidases degrading oligo-saccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). Purpose: To evaluate the hexosaminidase B (HEX B) activity in the saliva of children with type 1 diabetes. Materials and methods: The study was performed in 35 children with type 1 diabetes and 20 healthy children. Salivary HEX B activity was determined by the colorimetric, and protein by bicinchoninic acid methods. The HEX B activity concentration was expressed in pKat/mL and specific activity in pKat/µg of protein. Results: A significant increase in the concentration and the specific activity of HEX B in the saliva of children with type 1 diabetes, as compared to healthy children, was found. Conclusion: Children suffering from type 1 diabetes have increased catabolism of salivary gly-coconjugates by HEX B, which potentially may be useful in the diagnosis of type 1 diabetes in children.
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