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Effect of rhIL-17 on the expression of TLR2 and apoptotic proteins in human neutrophils

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Kakareko M., Jabłońska E., Jabłoński J., Ratajczak-Wrona W.
Medical Review
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2013
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issue 1
95-105
EN
Introduction. Neutrophils (PMNs) apoptosis occurs involving proteins of the Bcl-2 family, which control the permeability of the outer mitochondrial membrane. Proteins of this family are regulated by various factors, among others cytokine. Early observations of their own show the relationship between increased apoptosis and expression of Toll-like receptor (TLR2) in these cells stimulated by IL-17. The aim of the planned study was to investigate the effect of rhIL-17 on selected proteins of the Bcl-2 family, such as: the proapoptotic Bax protein and antiapoptotic Mcl-1 protein in relation to the expression of TLR2 receptor. Reference to the analysed results was to investigate the influence of fMLP on estimated parameters. Material and method. The study was conducted on human PMN isolated from peripheral blood. Apoptosis was assessed by cytometry, and the expression of TLR2, Bax, and Mcl-1 was estimated by Western blot. The results and conclusions. The results have shown that the enhancement of neutrophils intense apoptosis depends directly from the effect of rhIL-17 and probably indirectly on TLR2 receptor. The observed changes in the expression of Bax both under the influence of fMLP and rhIL-17 stress the important role of this protein in regulating neutrophils apoptosis.
PL
Wstęp. Apoptoza neutrofilów (PMN) zachodzi z udziałem białek rodziny Bcl-2, które kontrolują przepuszczalność zewnętrznej błony mitochondrialnej. Białka tej rodziny podlegają regulacji przez różne czynniki, m.in. cytokiny. Wcześniejsze obserwacje własne wskazują na relację między nasileniem apoptozy a ekspresją receptora TLR2 w komórkach stymulowanych IL-17. Celem zaplanowanych badań było zbadanie wpływu rhIL-17 na wybrane białka rodziny Bcl-2 szlaku wewnątrzkomórkowego, takich jak: proapoptotyczne białko Bax i antyapoptotyczne białko Mcl-1 w relacji do ekspresji receptora TLR2. Odniesieniem do analizowanych wyników było zbadanie wpływu fMLP na oceniane parametry. Materiał i metoda. Badania przeprowadzono na izolowanych z krwi obwodowej ludzkich PMN. Apoptozę oceniano metodą cytometrii przepływowej, natomiast ekspresję TLR2, Bax oraz Mcl-1 oznaczano metodą Western blot. Wyniki i wnioski. Uzyskane wyniki wykazały, że nasilona apoptoza neutrofilów zależy w sposób bezpośredni od wpływu rhIL-17 na badane białka szklaku wewnętrznego i prawdopodobnie w sposób zależny od receptora TLR2. Obserwowane zmiany ekspresji Bax zarówno pod wpływem fMLP, jak i rhIL-17 podkreślają
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The expression of apoptosis-related proteins in patients with ulcerative colitis

99%
Niewiarowska K., Lebelt A., Gryko M., Pryczynicz A., Famulski W., Czyżewska J., Kemona A., Piekarska-Dymicka V., Jagodzińska D., Guzińska-Ustymowicz K.
Progress in Health Sciences
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2014
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vol. 4
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issue 1
75-82
XX
Purpose: Recent literature data indicate a key role of apoptosis in the pathogenesis of inflammatory bowel disease. The aim of the study was to evaluate the expression of Bax, Bid, Bcl-2 and Bcl-xl in non-dysplastic and dysplastic epithelium in inflamated mucosa of patients with ulcerative colitis. Methods: The study consists of 18 patients with diagnosed ulcerative colitis. The expression of proteins was determined immunohistochemically. Results: Lack of Bax expression in normal epithelium of the inflamed intestinal mucosa (94.4%) and a weak expression of this protein were found in dysplastic glandular cells (67%). The Bax expression of dysplastic epithelium correlates with reduced severity of chronic inflammation (p<0.005). Bid expression in non-dysplastic glands was found in 67% of cases vs. 16% in dysplastic epithelium that was associated with the occurrence of epithelial erosions or ulcers (p<0.05). Moderate cytoplasmic expression of Bcl-xl was noted in 27.7% of patients in normal epithelium and in 66.1% within dysplastic lesions. Bcl-xl expression in dysplastic glandular cells correlated with the presence of neutrophils in the lamina propria (p <0.05). Conclusions: The immunohistochemical expressions of Bax, Bcl-2 and Bcl-xl increase and Bid protein expression decreases in dysplastic glandular tubes as compared to non-dysplastic intestinal epithelium in inflamed mucosa, which may suggest an imbalance of controlled cell death in ulcerative colitis.
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