The method of in vitro cultures is a specific technique of cultivation and study of plant material under sterile conditions. Plant tissue culture uses the plant’s ability of regeneration of the whole plant from its parts, even from a single cell. The culture is maintained in proper conditions of light and temperature that are different for plant species. Properties of the plant material and composition of physical properties of the medium have a profound impact of the quality of culture. In vitro plant cultures are widely used in plant science and find its application in practice in micropropagation, protection of biological diversity by creating banks of seeds, biotransformation, plant natural products or by creating transgenic organisms.
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