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Antimicrobial effects of propolis on Clostridium difficile strains belonging to the different PCR-ribotypes

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Piotrowski M., Pituch H., Obuch-Woszczatyński P.
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
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2016
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vol. 71
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issue 2
EN
Clostridium difficile is a Gram-positive, anaerobic rod-shaped bacterium, which is a primary cause of nosocomial diarrhoea. The rising incidence of antibiotic resistance in pathogens such as C. difficile makes the new antibacterial natural products research very important and necessary to conduct. It has been observed that propolis has bactericidal effects. Propolis is a natural resinous product that is manufactured by honeybees (Apis mellifera). The antimicrobial activity of propolis is an important biological property. In spite of the large composition differences of the propolis types, all of them have antimicrobial activity. Twenty strains of the C.difficile belonging to four prominent PCR-ribotypes (RT) (RT017, RT023, RT027, and RT046) were used in research. MBC value were determined by broth dilution method. Propolis samples were obtained from honey bees farm in lubelskie woyevodship. Bactericidal effect of the propolis on C.difficile strains was observed at its concentration of  39 mg/ml.
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