Application of Helicobacter pylori antigen test to evaluate gastric mucosa specimens

• Authors: Namiot, A , Leszczyńska, K , Namiot, DB , Chilewicz, M , Bucki, R , Kemona, A , Namiot, Z

Abstracts

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Purpose: To investigate, whether the test documenting the presence of Helicobacter pylori (H. pylori) antigens in the gastric mucosa may be used as diagnostic test. Materials and methods: Mucosal specimens taken from eighty-three patients during gastroscopic examination were subjected to rapid urease test (CLO test), histology, and H. pylori culture. The same biopsy specimens that had been evaluated in the CLO test or collected into the transport medium for bacterial culture were used to detect H. pylori antigens. An amplified immunoassay for the detection of H. pylori antigens in stool was used for gastric mucosa specimens. The sensitivity and specificity of the H. pylori antigen test were evaluated in relation to the results of each verifying test (CLO test, histology, culture) separately and to all 3 tests analysed together. Results: The sensitivity and specificity of the H. pylori antigen test in relation to the CLO test, histological examination, and H. pylori culture were 85.4% and 90.5%, 76.1% and 83.4%, and 90.7% and 90.0% for specimens taken for the CLO test and 90.0% and 82.0%, 78.0% and 81.0%, and 93.0% and 88.0% for specimens taken for bacterial culture, respectively. The sensitivity and specificity of the antigen test in relation to all 3 verifying tests analysed together were 91.3% and 97.3% for specimens taken for the CLO test, and 91.7% and 97.1% for specimens taken for bacterial culture, respectively. Conclusions: H. pylori antigen test in gastric mucosa specimens may be a sufficiently reliable source of information about stomach infection.

Keywords

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Helicobacter pylori antigens; immunoassay; gastric mucosa

• Publisher: Uniwersytet Medyczny w Białymstoku
• Journal: Progress in Health Sciences
• Year: 2014
• Volume: 4
• Issue: 2
• Pages: 52-57

Contributors

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Namiot, A

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Leszczyńska, K

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Namiot, DB

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Chilewicz, M

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Bucki, R

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Kemona, A

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Namiot, Z