Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil

• Authors: Michał Dąbrowski , Monika Sienkiewicz , Hanna Zielińska-Bliźniewska , Marta Dąbrowska , Małgorzata Seredyńska , Ewa Kochan
• Languages of publication: EN

Abstracts

EN

Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norfloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the efficacy of new agents to inactivate bacteria in clinical settings.

Keywords

EN

Escherichia coli, clove oil, urinary tract infections

• Publisher: Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
• Journal: Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
• Year: 2016
• Volume: 71
• Issue: 2

Dates

published

2016

online

2018-02-21

Contributors

author

Michał Dąbrowski

author

Monika Sienkiewicz

author

Hanna Zielińska-Bliźniewska

author

Marta Dąbrowska

author

Małgorzata Seredyńska

author

Ewa Kochan

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Identifiers

DOI

10.17951/c.2016.71.2.41