Medieval liturgical sources from various monastic communities are an important testimony to the monastic musical culture of the area. Musical prayer formed an essential part of the monk’s daily life. In addition to the Mass liturgy, which is also encountered in diocesan settings, the liturgy of the hours, which was celebrated essentially in the form of chant, determined the basic structure of the day for each member of the order. The aim of this study is to investigate the medieval liturgical chants of the Office for the Dead in Carthusian manuscripts from the territory of Moravia. Attention will be paid to selected chants of the Matins from these codices, primarily its responsories. These chants represent a specific, Carthusian liturgical tradition, which has been documented in its stable structure since the foundation of the Carthusian Order.