Inhibition of 12/15-LOX activity and ceramide pattern in the murine brain

• Authors: Fiedorowicz, A , Car, H , Prokopiuk, S , Sacharzewska, E , Żendzian-Piotrowska, M , Kowal, K

Abstracts

EN

Purpose: The 12/15-lipoxygenase (12/15-LOX) activity and the ceramide content are often elevated in neurodegenerative disorders; however their relationship in the brain is not established. To verify whether blocking of 12/15-LOX activity has an impact on ceramide pattern in the brain we inhibited the 12/15-LOX expression by administration of baicalein in mice. Materials and methods: The ceramides containing fatty acid methyl esters were analyzed using gas–liquid chromatography (GLC) technique. Results: The total ceramide content increased in baicalein-treated animals comparing to controls and the levels of most ceramide-fatty acids of SAFA, MUFA and PUFA tended to increase in relation to control. Baicalein treatment up-regulated significantly only the ceramide-lignoceric fatty acid in relation to controls. Conclusion: We have shown in this study that 12/15-LOX inhibition slightly alters the pattern of ceramides in the mouse brain.

Keywords

EN

12/15-lipoxygenase; ceramide; baicalein; mice

• Publisher: Uniwersytet Medyczny w Białymstoku
• Journal: Progress in Health Sciences
• Year: 2013
• Volume: 3
• Issue: 1
• Pages: 33-38

Contributors

author

Fiedorowicz, A

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Car, H

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Prokopiuk, S

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Sacharzewska, E

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Żendzian-Piotrowska, M

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Kowal, K