The influence of oxalate decarboxylase on the urinary oxalate excretion in swine model of nephrocalcinosis induced by hydroxyproline

• Authors: Katerina Pierzynowska , Stefan G. Pierzynowski , Liudamyla Lozinska , Sara Jarmakiewicz , Paulina Świeboda , Olexandr Fedkiv , Katarzyna Szwiec , Jose Louis Valverde Piedra , Rafal Filip
• Languages of publication: EN

Abstracts

EN

Introduction. Kidney stone formation may be a result of increased urinary oxalate supersaturation. Material and Methods. Eighteen pigs were randomly divided into: Control group, where standard cereal-based feed was supplemented with 4% HP only, Prevention group, where treatment with OxDc slurry started at the end of the adaptation period when pigs were switched to 4% HP diet, Reduction group, where the treatment with OxDc lyo powder started after pigs were already on a 4% HP diet for 6 days. Results. OxDc slurry prevented oxalate excretion in urine. The reduction effect of OxDc lyo feed addition was generally visible during the first two days of the therapy (p<0.05). Both dietary intake of 4% HP and OxDc preparations did not influence weight gain, water or feed intake, urine excretion and creatinine clearance. Conclusions. The capacity of OxDc in preventing induced hyperoxaluria was moderate. Most probably, this is due to the incoherent response of animals to the HP enriched diet dependent on their gut pH, since optimum pH for OxDc is around 5-6. A higher pH essentially reduces the activity of OxDc. The capacity of OxDc in reversing the hyperoxaluria induced by a HP enriched diet was significant during the first 2 days after introducing OxDc to the diet.

Keywords

EN

Nephrocalcinosis; Oxalate decarboxylase; Hydroxyproline; Pig model

• Publisher: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
• Journal: European Journal of Clinical and Experimental Medicine
• Year: 2017
• Issue: 3
• Pages: 206-2016

Contributors

author

Katerina Pierzynowska

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden
• Anara AB &SGPlus, Alfågelgranden 24, 23132 Trelleborg, Sweden

author

Stefan G. Pierzynowski

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden
• Anara AB &SGPlus, Alfågelgranden 24, 23132 Trelleborg, Sweden
• Department of Medical Biology, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland

author

Liudamyla Lozinska

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden
• Anara AB &SGPlus, Alfågelgranden 24, 23132 Trelleborg, Sweden

author

Sara Jarmakiewicz

• Department of Gastroenterology with IBD Unit of Clinical Hospital 2 Lwowska 60, Poland affiliated to the University of Rzeszow, Poland

author

Paulina Świeboda

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden

author

Olexandr Fedkiv

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden

author

Katarzyna Szwiec

• Department of Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden

author

Jose Louis Valverde Piedra

• Department of Medical Biology, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland
• Department of Biochemistry and Animal Physiology, University of Life Sciences, 20-950 Lublin, Poland

author

Rafal Filip

• Department of Biochemistry and Animal Physiology, University of Life Sciences, 20-950 Lublin, Poland
• Department of Gastroenterology with IBD Unit of Clinical Hospital 2 Lwowska 60, Poland affiliated to the University of Rzeszow, Poland

Identifiers

DOI

10.15584/ejcem.2017.3.4