Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis

• Authors: Magdalena Kutwin , Monika Migdalska-Sęk , Ewa Brzeziańska-Lasota , Piotr Zelga , Anna Woźniacka

Abstracts

EN

ObjectivesAs a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors – improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ).Material and MethodsThe obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B, IL-22 and IFN-γ genes, and presented as the relative quantification (RQ) value, relative to the reference gene GAPDH. In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life Quality Index (DLQI) scores was performed.ResultsStatistical analysis confirmed a significant increase in RQ values for IL-12B, IL-22 and IFN-γ in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene.ConclusionsIncreased expression levels of IL-12B, IL-22 and IFN-γ genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis.

Keywords

EN

psoriasis; disease activity; proinflammatory cytokines; skin disease; immunology; molecular markers

• Publisher: Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
• Journal: International Journal of Occupational Medicine and Environmental Health
• Year: 2020
• Volume: 33
• Issue: 5
• Pages: 635-647

Dates

published

2020

Contributors

author

Magdalena Kutwin

• Medical University of Lodz, Łódź, Poland (Department of Dermatology and Venereology)

author

Monika Migdalska-Sęk

• Medical University of Lodz, Łódź, Poland (Department of Biomedicine and Genetics)

author

Ewa Brzeziańska-Lasota

• Medical University of Lodz, Łódź, Poland (Department of Biomedicine and Genetics)

author

Piotr Zelga

• Medical University of Lodz, Łódź, Poland (Department of General and Colorectal Surgery)

author

Anna Woźniacka

• Medical University of Lodz, Łódź, Poland (Department of Dermatology and Venereology)

Identifiers

DOI

10.13075/ijomeh.1896.01513

Biblioteka Nauki

2116535