Effect of high fat diet on structure of liver and gallbladder of adult male mice – an experimental study

• Authors: Abdelmonem Awad Hegazy , Noura M. Qenawy , Nada M. Abdel Aziz , Emtethal M. El-Bestawy

Abstracts

EN

Introduction. High fat diet (HFD) intake induces obesity and adversely affects different body organs including liver and gallbladder. Aim. It was to clarify the effects of HFD on the liver and gallbladder structure using light microscopic (LM) examination. Material and methods. 16 healthy adult male mice were equally divided into 2 groups. Control group mice were fed normal diet. HFD group was fed using HFD. At the end of the 8-week experiment, mice were anesthetized. Liver and gallbladder were removed and prepared to histological processing. Sections were stained with hematoxylin and eosin (H&E) and immunostaining for cyclooxygenase-2 (COX-2) cellular localization. Oil Red O (ORO)-stained frozen liver sections were prepared. Results. H&E-stained sections of HFD group revealed rounded swollen hepatic cells with pale cytoplasm suggesting cellular ballooning. Dilated congested sinusoids and portal vein, cellular degeneration and collection of inflammatory cells were observed between hepatic cells and in portal region. Gallbladder sections showed epithelial stratification and cellular vacuolation. Strong immunoexpression of COX-2 was observed in Kupffer and hepatic cells of the liver and gallbladder mucosal epithelial cells. Conclusion. HFD is suggested to alter the normal histological features of liver and gallbladder represented by fatty liver and gallbladder epithelial hyperplasia and inflammatory reaction.

Keywords

EN

COX-2; fatty liver; gallbladder epithelium; immunoexpression; Oil Red O staining

• Publisher: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
• Journal: European Journal of Clinical and Experimental Medicine
• Year: 2021
• Issue: 4
• Pages: 291-298

Dates

published

2021

Contributors

author

Abdelmonem Awad Hegazy

• Human Anatomy & Embryology Department, Faculty of Medicine, Zagazig University, Zagazig City, Egypt
• Medical Biotechnology Department, College of Biotechnology, Misr University for Science and Technology (MUST), 6th of October City, Egypt

author

Noura M. Qenawy

• Human Anatomy & Embryology Department, Faculty of Medicine, Zagazig University, Zagazig City, Egypt

author

Nada M. Abdel Aziz

• Human Anatomy & Embryology Department, Faculty of Medicine, Zagazig University, Zagazig City, Egypt

author

Emtethal M. El-Bestawy

• Human Anatomy & Embryology Department, Faculty of Medicine, Zagazig University, Zagazig City, Egypt

Identifiers

DOI

10.15584/ejcem.2021.4.1

Biblioteka Nauki

2040247