A study on the mycobacterial burden and phenotypic drug resistance pattern with reference to the GeneXpert Cycle Threshold values in pulmonary tuberculosis

• Authors: Bineeta Kashyap , Krishna Sarkar , Kapil Singh , Puneeta Hyanki

Abstracts

EN

Introduction and aim. Tuberculosis (TB) remains a significant global health challenge. Early and accurate diagnosis is crucial to prevent further transmission. The present study aimed to correlate cycle threshold values with smear microscopy and culture positivity, and determine cut-off cycle threshold values for levels of smear grade and culture positivity. Material and methods. Forty presumptive cases of pulmonary TB were included and subjected to Ziehl-Neelsen stain, culture on Lowenstein Jensen media, CBNAAT and drug susceptibility testing for first line anti-tubercular drugs. Results. Our study predicts 3+, 2+, and 1+ sputum smear grade at a cut-off of Ct value ≤ 16.74, ≤19.68, and ≤ 22.32 respectively. A strong positive correlation was found between time to culture positivity and Ct value. A cut-off of Ct value ≤22.32 predicts culture positivity with a sensitivity of 92%, and a specificity of 67%. None of the isolates showed rifampicin resistance by 1% proportion method. Conclusion. Understanding the appropriate utilization of CBNAAT Ct values and their correlation with smear microscopy grade, culture, and drug susceptibility testing can assist clinicians in early identification and prompt initiation of appropriate treatment. This knowledge can contribute to the prevention of drug resistance, reduced transmission, and a decreased disease burden associated with TB.

Keywords

EN

CBNAAT; cycle threshold values; rifampicin resistance; smear microscopy; tuberculosis

• Publisher: Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
• Journal: European Journal of Clinical and Experimental Medicine
• Year: 2023
• Issue: 4
• Pages: 730-735

Dates

published

2023

Contributors

author

Bineeta Kashyap

• Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

author

Krishna Sarkar

• Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

author

Kapil Singh

• Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

author

Puneeta Hyanki

• DOTS Center, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

Identifiers

DOI

10.15584/ejcem.2023.4.11

Biblioteka Nauki

29520459